仪器资讯
Goat Anti-P14ARF抗体说明书
CatalogNumber: RS-1174R Quantitysize: 0.1ml （dilutewithpH7.4 0.01MPBSorantibodydiluent） Anti-P14ARF抗体说明书Background:Thisgenegeneratesseveraltranscriptvariantswhichdifferintheirfirstexons.Atleastthreealternativelysplicedvariantsencodingdistinctproteinshavebeenreported,twoofwhichencodestructurallyrelatedisoformsknowntofunctionasinhibitorsofCDK4kinase.Theremainingtranscriptincludesanalternatefirstexonlocated20Kbupstreamoftheremainderofthegene;thistranscriptcontainsanalternateopenreadingframe（ARF）thatspecifiesaproteinwhichisstructurallyunrelatedtotheproductsoftheothervariants.ThisARFproductfunctionsasastabilizerofthetumorsuppressorproteinp53asitcaninteractwith,andsequester,MDM1,aproteinresponsibleforthedegradationofp53.Inspiteofthestructuralandfunctionaldifferences,theCDKinhibitorisoformsandtheARFproductencodedbythisgene,throughtheregulatoryrolesofCDK4andp53incellcycleG1progression,shareacommonfunctionalityincellcycleG1control.Thisgeneisfrequentlymutatedordeletedinawidevarietyoftumors,andisknowntobeanimportanttumorsuppressorgene.CDKN2AAnti-P14ARF抗体说明书pecificity: – Anti-P14ARFisagoatmonoclonalantibodyunconjugated – specificforP14ARFofrabbit – useforwesternblotting,elisa,immunoprecipitationandimmunohistochemistry– ProteinGaffinitychromatographypurification,purity:>95%– Isotype: IgG– molwt: 12.5kDa Application: – Westernblotting1:100-500 – Immunohistochemistry1:100-500 – ELISA1:500-1000 – Optimalworkingdilutionsmustbedeterminedbytheenduser. Anti-P14ARF抗体说明书Storage: Storeat-20℃foroneyear.Avoidrepeatedfreeze/thawcycles.The lyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreater thanayearwhenkeptat-20℃.WhenreconstitutedinsterilepH7.40.01MPBSordiluent ofantibody,theantibodyisstableforatleastsixweeksat2-4℃ ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforusein human,therapeuticordiagnosticapplications.
