SFC VCD振动圆二色光谱仪测量蛋白质

It is generally understood that chiral compounds have different bioactivities depending upon the absolute
configuration of each compound. Some familiar examples include glutamic acid and thalidomide. Lglutamic
acid demonstrates the Umami taste*1, while D-glutamic acid has a bitter taste, similarly, the R
form of thalidomide is a sedative, but the S form has teratogenic activity. Thus, the separation and study
of chiral compounds is critical for many reasons.
The functionalities of chiral compounds have been studied for the development of advanced molecules
for many applications. The study of chiral compounds has spread to several fields such as natural
products, pharmaceuticals and other functional molecules, and it can be pointed out that among those
studies, the structural analysis of chiral compounds is a very important topic. X-ray Diffraction (XRD),
Nuclear Magnetic Resonance (NMR) and Electronic Circular Dichroism (ECD) using UV/Vis light are
employed as primary methods for the structural analysis of chiral compounds. In this paper, the
measurement of chiral compounds by Vibrational Circular Dichroism (VCD) using infrared light will be
outlined.
VCD is a method to measure the difference of absorbance intensity between left-hand and right-hand
circularly polarized light as shown in Figure 1. It is an advantage of VCD that this method can be applied
to almost all organic compounds in the same way as infrared (IR) spectroscopy. In addition, by
comparing the measurement results with calculated results by ab-initio molecular orbital calculations, the
absolute configuration of the sample can be determined. However, since the peak intensity of VCD
spectra are 1,000 10,000 times weaker than that of standard IR spectra, spectroscopic instruments
with high sensitivity and stability with very small baseline fluctuations are required. The FVS-6000 VCD
system has a high sensitivity detector, suitable optical JASCO振动圆二色光谱仪VCD FVS-6000