采用 Agilent AssayMAP 自动进行细胞表面蛋白质分离

This study investigated the feasibility of automating an established cell surface
protein isolation protocol using the Agilent AssayMAP Bravo automation platform.
Epidermoid cancer cell lines
-
A431 was used as an
in
-
vitro
model system, and
cell surface proteins were isolated using a combination of AssayMAP Bravo
automation and a Pier
ce Cell Surface Protein Isolation kit. The isolated proteins
were then analyzed using the Agilent 2200 TapeStation system in conjunction
with the Agilent P200 ScreenTape assay for differential electrophoresis profi le.
The eluted proteins were then anal
yzed using the Agilent HPLC
-
chip coupled to
an Agilent 6550 iFunnel Q
-
TOF with dual stage ion funnel technology. Key cell
surface proteins were identifi ed including extracellular matrix proteins, moderately
abundant proteins including pancreatic marker
protein, plectin
-
1 along with F
-
box
leucine rich repeat protein
-
2,
beta
-
actin, and PGK
-
2. This study demonstrates
that the AssayMAP Bravo platform can be conveniently used for automated
high
-
throughput sample preparation workfl ows involving affi nit
y purifi cations 6500 系列四极杆(Q-TOF) 液质联用系统