蛋白质药物质谱应用文集

The Q Exactive MS is well suited for targeted quantification
providing two powerful HR/AM approaches, msx tSIM and tHCD. Both approaches demonstrate high selectivity, high sensitivity and high throughput due to several hardware
and software innovations.
? For the msx tSIM approach, high mass accuracy (< 5 ppm)
and high resolution (140K) ensures accurate identification
of target ions in the presence of complex background, while
spectrum multiplexing dramatically increases throughput without sacrificing resolution.
? A LOD of 10 amol and 4-order linear dynamic range was obtained for most peptides with msx tSIM method in the presence of medium complex background.
? Different from triple-quadrupole-based SRM, all fragment ions are simultaneously detected with high mass accuracy and high resolution using the tHCD approach. The HR/AM of fragment ions provides unmatched selectivity. The simultaneous detection of all fragment ions allows more efficient samp领 of target peptides and results in improved sensitivity. A scan speed of 12 Hz at a resolution of 17,500 ensures high throughput.
? A LOD of 50 amol and 3-4 orders linear dynamic range was obtained for most peptides with tHCD method in the presence of strong complex background. 赛默飞Q Exactive Orbitrap LCMSMS系统 赛默飞TSQ Endura三重四极杆质谱仪 赛默飞TSQ Quantiva 三重四极杆质谱仪 赛默飞Q Exactive Focus LCMSMS 系统 赛默飞LTQ Orbitrap XL组合式质谱仪