仪器资讯
RabbitAnti-Vimentin抗体流式说明书,FITCconjugated
CatalogNumber:RSF-0272R
Quantitysize:100ug（2mg/ml,Lyophilized.Buffer=0.01MPBS,pH7.4with10mg/mlBSAand0.1%Sodiumazide.）
Background:Vimentinisthemajorsubunitproteinoftheintermediatefilamentsofmesenchymalcells.Itisbelievedtobeinvolvedwiththeintracellulartransportofproteinsbetweenthenucleusandplasmamembrane.Vimentinhasbeenimplicatedtobeinvolvedintherateofsteroidsynthesisviaitsroleasastoragenetworkforsteroidogeniccholesterolcontaininglipiddroplets.VimentinphosphorylationbyaproteinkinasecausesthebreakdownofintermediatefilamentsandactivationofanATPandmyosinlightchain-dependentcontractileevent.Thisresultsincytoskeletalchangesthatfacilitatetheinteractionofthelipiddropletswithinmitochondria,andsubsequenttransportofcholesteroltotheorganellesleadingtoanincreaseinsteroidsynthesis.ImmunohistochemicalstainingforVimentinischaracteristicofsarcomas（ofneural,muscleandfibroblastorigin）comparedwithcarcinomaswhicharegenerallynegative.Melanomas,lymphomasandvasculartumorsmayallstainforVimentin.Vimentinantibodiesarethusofvalueinthedifferentialdiagnosisofundifferentiatedneoplasmsandmalignanttumors.Theyaregenerallyusedwithapanelofotherantibodiesincludingthoserecognizingcytokeratins,lymphoidmarkers,S100,desminandneurofilaments.
RabbitAnti-Vimentin抗体流式说明书,FITCconjugated
Specificity:•anti-Vimentinisarabbitmonoclonalantibody,FITCconjugated
•specificforVimentinofGoat,mouseandrat
•ProteinGaffinitychromatographypurification,purity:>95%
•Isotype:IgG
•molwt：95kDa
Application:•FCM: 1:20-100
•Immunohistochemistry:1:20-1:200
•Immunocytochemistry:1:20-1:200
•Excitationspectrum:490-495nm
•Emissionspectrum:520-530nm
•Optimalworkingdilutionsmustbedeterminedbytheenduser.
•protectfromlight.
RabbitAnti-Vimentin抗体流式说明书,FITCconjugated
Storage:Storeat–20ºCforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20ºC.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibody,theantibodyisstableforatleasttwoweeksat2-4ºC.
ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.
RabbitAnti-Vimentin抗体流式说明书,FITCconjugated
