P53抗体antibody说明书

Rabbit Anti-

CatalogNumber:Rs-2090RQuantitysize:  100ul （dilutewithpH7.4 0.01MPBSorantibodydiluent）Background: ThewidelystudiedP53tumorsuppressorgenehasbeenfoundtocontainmutationsinover50%ofhumancancers.ThelevelofP53proteinislowinnormalcells,butisincreasedinresponsetoDNAdamageorvariousothercellulardistresssignals.OverexpressionoftheP53transcriptionfactorcaninduceethercellcyclearrestorapoptosisthroughtranscriptionalregulationofseveralgenesincludingthecellcycleingibitorP21,DNArepairgeneGADD45,andtheapoptoticinducerBax.P53hasalsobeenshowntoinduceapoptosisbymeasofsdirectsigna领pathway.P53directlybindstoandactsonseveralcellularproteininvolvedinvariouspathway,includingc-Abl,basaltranscriptionfactorTFIIHandWT1.P53canbefunctionallyinactivatedbymutation,bindingtoDNAtumorvirusencodedproteins,suchasSV40largeTantigen,AdenovirusE1BandpapillomavirusE6proteinsorasaconsequenceofitsinteractionwiththeoncogene-encodedproteinMDM2.             Specificity:       ·anti-P53 isaRabbitmonoclonalantibodyunconjugated ·specificforP53of chiken·useforwesternblotting,elisa,immunoprecipitation  andimmunohistochemistry· ProteinGaffinitychromatographypurification,purity:>95%·Isotype:IgG· molwt:53kDa                

Rabbit Anti-

Application:      ·Westernblotting1:100-500·Immunohistochemistry1:100-500·ELISA1:500-1000· Optimalworkingdilutionsmustbedeterminedbytheenduser. Storage:    Storeat–20ºCforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20ºC.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibody,theantibodyisstableforatleasttwoweeksat2-4ºC. ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.

Rabbit Anti-

  
2004-07-13