Anti-IL-8/CXCL8抗体说明书WBIHC
Anti-IL-8/CXCL8抗体说明书,拥有免疫组化wb技术From:ABCAM CatalogNumber:RS-0780RQuantitysize: 0.1ml (dilutewithpH7.4 0.01MPBSorantibodydiluent)Background:IL-8isachemotacticfactorthatattractsneutrophils,basophils,andT-cells,butnotmonocytes.Itisalsoinvolvedinneutrophilactivation.Itisreleasedfromseveralcelltypesinresponsetoaninflammatorystimulus.IL-8(6-77)hasa5-10-foldhigheractivityonneutrophilactivation,IL-8(5-77)hasincreasedactivityonneutrophilactivationandIL-8(7-77)hasahigheraffinitytoreceptorsCXCR1andCXCR2ascomparedtoIL-8(1-77),respectively.Belongstotheintercrinealpha(chemokineCxC)family.
Function:IL-8isachemotacticfactorthatattractsneutrophils,basophils,andT-cells,butnotmonocytes.Itisalsoinvolvedinneutrophilactivation.Itisreleasedfromseveralcelltypesinresponsetoaninflammatorystimulus.IL-8(6-77)hasa5-10-foldhigheractivityonneutrophilactivation,IL-8(5-77)hasincreasedactivityonneutrophilactivationandIL-8(7-77)hasahigheraffinitytoreceptorsCXCR1andCXCR2ascomparedtoIL-8(1-77),respectively.
Post-translationalmodifications:SeveralN-terminalprocessedformsareproducedbyproteolyticcleavageaftersecretionfromatleastperipheralbloodmonocytes,leukcocytesandendothelialcells.Ingeneral,IL-8(1-77)isreferredtoasinterleukin-8.IL-8(6-77)isthemostpromimentform..Specificity: Anti-IL-8isamousemonoclonalantibody specificfor IL-8ofhu– useforwesternblotting,elisa,immunoprecipitationandimmunohistochemistry– ProteinGaffinitychromatographypurification,purity:>95%– Isotype: IgG– molwt: 11kDaAnti-IL-8/CXCL8抗体说明书,拥有免疫组化wb技术Application: – WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500– KLHconjugatedsyntheticpeptidederivedfromhumanIL-8C-terminus– notyettestedinotherapplications.
optimaldilutions/concentrations首ldbedeterminedbytheenduser..Storage: Storeat-20℃foroneyear.Avoidrepeatedfreeze/thawcycles.The lyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreater thanayearwhenkeptat-20℃.WhenreconstitutedinsterilepH7.40.01MPBSordiluent ofantibody,theantibodyisstableforatleastsixweeksat2-4℃ ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforusein human,therapeuticordiagnosticapplications.Anti-IL-8/CXCL8抗体说明书,拥有免疫组化wb技术
2004-07-13