Super Script Ⅱ阴性逆转录酶,SuperScript II Reverse Transcriptase

文档简介SuperScriptⅡ阴性逆转录酶,SuperScriptIIReverseTranscriptase英文名称：SuperScriptIIReverseTranscriptase级别：BR来源：PurifiedfromE.coliexpressingthepolgeneofM-MLV(1,2),mutagenizedtoreducetheRNaseHactivity浓度：200u/ul活力定义：OneunitofSuperScriptIIistheamountofenzymerequiredtoincorporate1nmoleofeoxyribonucleotideintoacid-precipitablematerialin10min.at37°Cusingpoly(A)oligo(dT)12-18astemplateprimer(3).UnitReactionConditions：50mMTris-HC1(pH8.3),40mMKC1,6mMMgC12,1mMDTT,0.5mM[3H]dTTP,0.1mMpoly(A),0.1mMoligo(dT)12-18,0.1mg/m1BSA,andenzymein50lfor10min.at37℃PerformanceandQualityTesting：SDS-PAGEpurity；endodeoxyribonuclease,exodeoxyribonuclease,andribonucleaseassays;andyieldandlengthofcDNAproductEnzymeFunction：RNAdependentDNAPolymeraseFinalProductSize：12.3kborlessNumberofReactions：10ReactionsOptimalReactionTemperature：42℃Sensitivity：MediumTemplate：ssRNA；ssDNA；RNA-DNAHybrid产品描述：SuperScriptIIReverseTranscriptase(RT)isanimprovedversionofSuperScriptRT.ItisaDNApolymerasethatsynthesizesacomplementaryDNAstrandfromsingle-strandedRNA,DNA,oranRNA:DNAhybrid.ThisenzymeisgeneticallyengineeredbytheintroductionofpointmutationsratherthanadeletionintheRNaseHactivecenter.LikeSuperScriptRT,SuperScriptIIRThasreducedRNaseHactivity.UnlikeSuperScriptRT,however,theselectivemutationswithintheRNaseHdomainmaintainfullpolymeraseactivity.ThisstructuralmodificationeliminatesdegradationofRNAmoleculesduringfirst-strandcDNAsynthesisandgivesSuperScriptIIRTsuperiorperformancecharacteristics,including：Greaterfirst-strandcDNAyields；Morefull-lengthcDNAsynthesis；Fullactivityat42℃性状：悬浮液用途：生化研究。保存：-20℃SuperScriptⅡ阴性逆转录酶,SuperScriptIIReverseTranscriptaseSuperScriptⅡ阴性逆转录酶,SuperScriptIIReverseTranscriptaseSuperScriptⅡ阴性逆转录酶,SuperScriptIIReverseTranscriptase