Super Script Ⅱ阴性逆转录酶技术报告

文档简介SuperScriptⅡ阴性逆转录酶技术报告大鼠血管活性肠肽(VIP)elisa试剂盒大鼠脱氢表雄酮S7(DHEA-S7)elisa试剂盒多腺苷二磷酸多聚酶抗体双花扁豆凝集素(DBA)elisa试剂盒可溶性载质转运蛋白SLC24A5抗体软骨糖蛋白39抗体淀粉抗体CD68抗体（人、牛）人β氨基己糖苷酶A(β-hexosaminidaseA)elisa试剂盒小鼠肿瘤坏死因子相关凋亡诱导配体1(TRAIL-R1)elisa试剂盒猪氧化低密度脂蛋白(OxLDL)elisa试剂盒抗ETag抗体SuperScriptⅡ阴性逆转录酶技术报告级别：BR来源：PurifiedfromE.coliexpressingthepolgeneofM-MLV(1,2),mutagenizedtoreducetheRNaseHactivity浓度：200u/ul活力定义：OneunitofSuperScript?IIistheamountofenzymerequiredtoincorporate1nmoleofeoxyribonucleotideintoacid-precipitablematerialin10min.at37°Cusingpoly(A)?oligo(dT)12-18astemplate?primer(3).UnitReactionConditions：50mMTris-HC1(pH8.3),40mMKC1,6mMMgC12,1mMDTT,0.5mM[3H]dTTP,0.1mMpoly(A),0.1mMoligo(dT)12-18,0.1mg/m1BSA,andenzymein50μlfor10min.at37℃PerformanceandQualityTesting：SDS-PAGEpurity；endodeoxyribonuclease,exodeoxyribonuclease,andribonucleaseassays;andyieldandlengthofcDNAproductEnzymeFunction：RNAdependentDNAPolymeraseFinalProductSize：12.3kborlessNumberofReactions：10ReactionsOptimalReactionTemperature：42℃Sensitivity：MediumTemplate：ssRNA；ssDNA；RNA-DNAHybrid产品描述：SuperScript?IIReverseTranscriptase(RT)isanimprovedversionofSuperScript?RT.ItisaDNApolymerasethatsynthesizesacomplementaryDNAstrandfromsingle-strandedRNA,DNA,oranRNA:DNAhybrid.ThisenzymeisgeneticallyengineeredbytheintroductionofpointmutationsratherthanadeletionintheRNaseHactivecenter.LikeSuperScript?RT,SuperScript?IIRThasreducedRNaseHactivity.UnlikeSuperScript?RT,however,theselectivemutationswithintheRNaseHdomainmaintainfullpolymeraseactivity.ThisstructuralmodificationeliminatesdegradationofRNAmoleculesduringfirst-strandcDNAsynthesisandgivesSuperScript?IIRTsuperiorperformancecharacteristics,including：Greaterfirst-strandcDNAyields；Morefull-lengthcDNAsynthesis；Fullactivityat42℃性状：悬浮液用途：生化研究。SuperScriptⅡ阴性逆转录酶技术报告