Super Script Ⅱ阴性逆转录酶参数报告

文档简介SuperScriptⅡ阴性逆转录酶参数报告性状：悬浮液用途：生化研究。保存：-20℃SuperScriptⅡ阴性逆转录酶参数报告英文名称：SuperScriptIIReverseTranscriptase级别：BR来源：PurifiedfromE.coliexpressingthepolgeneofM-MLV(1,2),mutagenizedtoreducetheRNaseHactivity浓度：200u/ul活力定义：OneunitofSuperScript?IIistheamountofenzymerequiredtoincorporate1nmoleofeoxyribonucleotideintoacid-precipitablematerialin10min.at37°Cusingpoly(A)?oligo(dT)12-18astemplate?primer(3).UnitReactionConditions：50mMTris-HC1(pH8.3),40mMKC1,6mMMgC12,1mMDTT,0.5mM[3H]dTTP,0.1mMpoly(A),0.1mMoligo(dT)12-18,0.1mg/m1BSA,andenzymein50μlfor10min.at37℃PerformanceandQualityTesting：SDS-PAGEpurity；endodeoxyribonuclease,exodeoxyribonuclease,andribonucleaseassays;andyieldandlengthofcDNAproductEnzymeFunction：RNAdependentDNAPolymeraseFinalProductSize：12.3kborlessNumberofReactions：10ReactionsOptimalReactionTemperature：42℃Sensitivity：MediumTemplate：ssRNA；ssDNA；RNA-DNAHybrid产品描述：SuperScript?IIReverseTranscriptase(RT)isanimprovedversionofSuperScript?RT.ItisaDNApolymerasethatsynthesizesacomplementaryDNAstrandfromsingle-strandedRNA,DNA,oranRNA:DNAhybrid.ThisenzymeisgeneticallyengineeredbytheintroductionofpointmutationsratherthanadeletionintheRNaseHactivecenter.LikeSuperScript?RT,SuperScript?IIRThasreducedRNaseHactivity.UnlikeSuperScript?RT,however,theselectivemutationswithintheRNaseHdomainmaintainfullpolymeraseactivity.ThisstructuralmodificationeliminatesdegradationofRNAmoleculesduringfirst-strandcDNAsynthesisandgivesSuperScript?IIRTsuperiorperformancecharacteristics,including：Greaterfirst-strandcDNAyields；Morefull-lengthcDNAsynthesis；Fullactivityat42℃SuperScriptⅡ阴性逆转录酶参数报告人丝裂原激活的蛋白激酶/MAP激酶(MAPK)ELISA试剂盒血栓调节蛋白elisa原理,大鼠TM/elisa步骤说明人胰岛细胞抗原2抗体/蛋白酪氨酸磷酸酶抗体elisa原理,人IA-2A/elisa步骤说明人麦角蛋白IgA(Gliadin-IgA)ELISA试剂盒兔抗单核细胞抗体实验步骤(AMA)elisa技术开发人可溶性凋亡相关因子(sFAS/Apo-1)ELISA试剂盒猫α干扰素实验步骤(IFN-α)elisa技术开发人巨噬细胞来源的趋化因子(MDC/CCL22)ELISA试剂盒牛孕激素/孕酮elisa原理,(PROG)elisa步骤说明