AnalyticalMethodsofVolatileAnalysisManyanalyticalmethodshavebeenusedforthesamplingandisolationofvolatilecompoundsgeneratedduringlipidoxidation.Sampling,usingmethodssuchas... Analytical Methods of Volatile Analysis Many analytical methods have been used for the sampling and isolation of volatile compounds generated during lipid oxidation. Sampling, using methods such as extraction, headspace analysis, and solid-phase microextraction (SPME), offers a means to collect and often pre-concentrate volatiles from samples. Isolation allows the separation of the extracted volatiles and is often accomplished
using gas chromatography (GC) or high-pressure liquid chroma-
tography (HPLC). Aromatic compounds are produced in low con- centrations at the onset of lipid oxidation, thereby requiring a method that not only isolates the compounds, but concentrates them. In addition, food provides a complex matrix from which to extract the compounds. Several studies have addressed the bind- ing of lipid oxidation products to proteins (O’Keefe and others 1991; Gutheil and others 1992). These bound compounds need to be liberated before the oxidation products can be accurately quantified. Sample preparation can complicate the assessment of lipid oxidative status as a number of these lipid oxidation prod- ucts are thermally generated. Care must be taken to extract the compounds present in the sample while not generating additional oxidation products or allowing the volatile compounds to de- grade during the extraction process.
The most common procedures reported in the literature for the determination of lipid oxidation products from muscle foods are GC methods. In this review, sampling methodologies of distillation and solvent extraction, and isolation methods associated with GC and HPLC analyses will be discussed. Within those gas chromato- graphic methodologies, static HS, dynamic purge-and-trap HS, and solid-phase microextraction (SPME) will be addressed. A summary of the different isolation methods is presented in Table 1.
只要翻译的通顺即可。虽然长了点,可是80分还是值得的吧