The most common means of agitation of liquid media is by placing the culture vessels on a drum(for test tubes)or a disk(for flasks)slowly rotating around a near horizontal axis. This assures good aeration of the liquid cultures with little... The most common means of agitation of liquid media is by placing the culture vessels on a drum(for test tubes)or a disk(for flasks)slowly rotating around a near horizontal axis. This assures good aeration of the liquid cultures with little mechanical damage to the cells.Agitation can also be achieved by placing culture flasks on reciprocating or gyrotory platform shakers.Gyrotoy (horizontal rotary orbit)shakers are preferred over reciprocating (horizontal linear motion)ones for a variety of reasons .The performance of the gyrotory shaker is determined by the speed of agitation and angle position of the flasks,with optimal aeration occurring in flasks tilted about 45° from the vertical.A new shaker design is the "tapping" motion shaker in which the culture is agitated by a magnetic bar moving up and down.This vertical movement of the bar is less injurious to cells than rotary movement of the bar at the bottom of the flask used in some conventional culture systems.
Z常见的手段,搅拌液体的媒体是把文化的船只鼓(试管)或磁盘(瓶) ,慢慢旋转周围附近的横轴。这保证了良好的曝气的液体文化与小机械损伤到cells.agitation也可以把所取得的文化瓶就往复或gyrotory平台的热点。gyrotoy (水平旋转的轨道)热点是shou选超过往复(横向直线运动) ,以便其原因有多种。表现的gyrotory振动筛是由高速搅拌和角度的立场,该瓶,与Z优曝气发生在瓶倾斜约45 °从vertical.a新的振动筛设计是“窃听”的议案,振动筛,其中文化是激动,由磁条移动向上和向下。这垂直运动大律师公会是少损害细胞比旋转运动的香港大律师公会在底部的瓶子用在一些传统的文化系统。