目的 制备足浴液并对其进行质量考察。方法 采用正交试验优选苦参中苦参碱和紫草中紫草素的提取工艺，通过紫外分光光度法对苦参碱与紫草素的含量进行测定，并将苦参碱、紫草素、水杨酸、苯甲酸和硼酸按一定比例进行溶解实验。化学成分分析采用薄层色谱法及试管... 目的 制备足浴液并对其进行质量考察。方法 采用正交试验优选苦参中苦参碱和紫草中紫草素的提取工艺，通过紫外分光光度法对苦参碱与紫草素的含量进行测定，并将苦参碱、紫草素、水杨酸、苯甲酸和硼酸按一定比例进行溶解实验。化学成分分析采用薄层色谱法及试管实验法对苦参中苦参碱与紫草中紫草素进行化学成分检测。结果 通过薄层色谱法和试管实验法得出苦参碱为生物碱、紫草素为蒽醌。通过正交试验和紫外分光光度法选出Z优提取方案。通过溶解实验使中化学药物形成混悬液，Z后得到足浴液样品。结论 通过T浓度实验，溶解实验选用95%乙醇为溶媒。通过L9(33)正交实验确定Z佳提取工艺，即提取时间为30min，温度为60℃，乙醇浓度为60%，以苦参碱作对照品，用分光光度法测定其苦参碱得率3.07%。经过实验摸索，得到超声提取紫草工艺的Z佳条件，即乙醇浓度为70%，超声功率为400w，料液比为1：40时提取率较高。足浴液制备方法简便，有利于实际生产，降低能源、节约时间，有较大的开发利用价值和广泛的市场前景。
关键字：紫草；苦参；正交设计；薄层色谱
Now ，preparation enough to shower and their quality inspection. Methods orthogonal test and optimization of Matrine Kushen Shikonin shikonin in the extraction process， through UV spectrophotometry of matrine and shikonin to determine the content and matrine， Shikonin Su， salicylic acid， benzoic acid and boric acid dissolved by a certain percentage to experiment. Chemical composition analysis by TLC and test-tube experiments in the Kushen Matrine and Shikonin shikonin in the chemical composition testing. Results TLC and test-tube experiments that matrine for alkaloids， shikonin for anthraquinone. By orthogonal testing and optimal UV spectrophotometry elected from the programme. Through the dissolution of the prescription drug in the form of suspension， enough to shower the end of samples. Conclusion T concentration of dissolved choice of 95 percent ethanol as solvent. Through L9 (33) orthogonal experiment to determine the optimal extraction process， that is， from time to 30 min， the temperature was 60 ℃， alcohol concentration of 60 percent， to matrine for reference substance， with its Determination in the matrine Rate of 3.07 percent. After experimental exploration， extraction by ultrasound Shikonin the best of conditions， namely the concentration of 70 percent ethanol， ultrasonic power for 400 w， feed than for the higher rate of extraction at 1:40. Preparation is simple enough to shower， to actual production， reduce energy and save time， have greater value and the development and utilization of broad market prospects.
Keyword: Shikonin; Kushen; orthogonal design; TLC
评论
好基123575 
长按识别二维码查看信息详情