白介素15(IL15)重组蛋白
Recombinant Interleukin 15 (IL15)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism speciesCavia (Guinea pig )Product No.yb061Gu01SourceProkaryotic expressionHostE.coliPurity> 95%UOM50ugPredicted Molecular Mass18.8kDaConcentration5.1ApplicationsSDS-PAGE; WB; ELISA; IP.Endotoxin Level<1.0EU per 1µg (determined by the LAL method)FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.白介素15(IL15)重组蛋白SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- SW HDVIYDLKRI ENLIQSIHID ATLYTESDVH PNCKITAMKC FLLELRVILH ESRNEDIHET ITNLIILANS SLNSNGNVTE SGCKECEELE EKSIAEFLQS FVHIVQMFIN TS
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate 白介素15(IL15)重组蛋白of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double 白介素15(IL15)重组蛋白intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.