胞外超氧化物歧化酶（SOD3）重组蛋白

胞外超氧化物歧化酶(SOD3)重组蛋白

RecombinantSuperoxideDismutase3,Extracellular(SOD3)

FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!

OrganismspeciesHomosapiens(Human)ProductNo.xyA117Hu01SourceProkaryoticexpressionHostE.coliPurity>95%UOM50ugPredictedMolecularMass27.9kDaConcentrationn/aApplicationsSDS-PAGE;WB;ELISA;IP.EndotoxinLevel<1.0EUper1g(determinedbytheLALmethod)ResiduesTrp19~Ala240(Accession#P08294)withtwoN-terminalTags,His-tagandT7-tag

FormulationSuppliedaslyophilizedformin20mMTris,150mMNaCl,pH8.0,containing1mMEDTA,1mMDTT,0.01%sarcosyl,5%trehalose,andpreservative.

胞外超氧化物歧化酶(SOD3)重组蛋白SEQUENCES

Thesequenceofthetargetproteinislistedbelow.
WTGEDSAEPNSDSAEWIRDMYAKVTEIWQEVMQRRDDDGALHAACQVQPSATLDAAQPRVTGVVLFRQLAPRAKLDAFFALEGFPTEPNSSSRAIHVHQFGDLSQGCESTGPHYNPLAVPHPQHPGDFGNFAVRDGSLWRYRAGLAASLAGPHSIVGRAVVVHAGEDDLGRGGNQASVENGNAGRRLACCVVGVCGPGLWERQAREHSERKKRRRESECKAA

胞外超氧化物歧化酶(SOD3)重组蛋白USAGE

ReconstituteinsterilePBS,pH7.2-pH7.4.

STORAGEANDSTABILITY

Storage:Avoidrepeatedfreeze/thawcycles.Storeat2-8oCforonemonth.Aliquotandstoreat-80oCfor12months.
StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37oCfor48h,andnoobviousdegradationandprecipitationwereobserved.(ReferringfromChinaBiologicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinislessthan5%withintheexpirationdateunderappropriatestoragecondition.

AbouttheMARKER(complimentary)

EffectiveSizeRange:10kDato70kDa.
Proteinbands:10kDa,14kDa,18kDa,22kDa,26kDa,33kDa,44kDaand胞外超氧化物歧化酶(SOD3)重组蛋白70kDa.
Doubleintensitybands:The26kDa,18kDa,10kDabandsareatdoubleintensitytomakelocationandsizeapproximationofproteinsofinterestquickandeasy.
Ready-to-use:Noneedtoheat,diluteoraddreducingagentsbeforeuse.

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2004-09-12