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C6 大鼠脑胶质瘤细胞

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C6大鼠脑胶质瘤细胞说明书

GeneralInformation:

Organism:

Rattusnorvegicus,rat

Tissue:

brain

CultureProperties:

adherent

Morphology:

fibroblast

CultureMethod:

CompleteGrowthMedium:

F-12K(Gibco21127-022)+2.5%FBS+15%horseserum

+Penicillin/Streptomycin

Subculturing:

Volumesaregivenfora25cm2flask.Increaseordecreasetheamountofdissociationmediumneededproportionallyforculturevesselsofothersizes.

Removeanddiscardculturemedium.Brieflyrinsethecelllayerwith0.25%(w/v)Trypsin-EDTAsolutiontoremovealltracesofserumthatcontainstrypsininhibitor.Add1.0to2.0mLofTrypsin-EDTAsolutiontoflaskandobservecellsunderaninvertedmicroscopeuntilcelllayerisdispersed.DiscardTrypsin-EDTAsolution.Add6.0to8.0mLofcompletegrowthmediumandaspiratecellsbygentlypipetting.Addappropriatealiquotsofthecellsuspensiontonewculturevessels.Incubateculturesat37°C,5%CO2.

SubcultivationRatio:

Asubcultivationratioof1:2to1:3isrecommended

Cryopreservation:

Freezemedium:Completegrowthmediumsupplementedwith10%(v/v)DMSO

Storagetemperature:liquidnitrogenvaporphase


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2004-12-04
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