转化生长因子β1（TGFβ1）检测试剂盒

专业评测

转化生长因子β1(TGFβ1)检测试剂盒

适用生物Chicken(Gallus，鸡)
转化生长因子β1(TGFβ1)检测试剂盒检测范围15.63-1000pg/mL灵敏度6.2pg/mL
样本类型Serum,plasmaandotherbiologicalfluids.
实验时长4.5h实验方法双抗夹心法转化生长因子β1(TGFβ1)检测试剂盒规格96T
ELISAKitforTransformingGrowthFactorBeta1(TGFb1)
FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!

OrganismspeciesChicken(Gallus)
转化生长因子β1(TGFβ1)检测试剂盒SampletypeSerum,plasmaandotherbiologicalfluids.
Format96-wellstripplate
Assaylength4.5hours
Detectionrange15.63-1000pg/mLThestandardcurveconcentrationsusedfortheELISA’swere1000pg/mL,500pg/mL,250pg/mL,125pg/mL,62.5pg/mL,31.25pg/mL,15.63pg/mL
SensitivityTheminimumdetectabledoseofthiskitistypicallylessthan6.2pg/mL.

Specificity
ThisassayhashighsensitivityandexcellentspecificityfordetectionofTransformingGrowthFactorBeta1(TGFb1).
Nosignificantcross-reactivityorinterferencebetweenTransformingGrowthFactorBeta1(TGFb1)andanalogueswasobserved.
Recovery
Matriceslistedbelowwerespiked转化生长因子β1(TGFβ1)检测试剂盒withcertainlevelofrecombinantTransformingGrowthFactorBeta1(TGFb1)andtherecoveryrateswerecalculatedbycomparingthemeasuredvaluetotheexpectedamountofTransformingGrowthFactorBeta1(TGFb1)insamples.

MatrixRecoveryrange(%)Average(%)
serum(n=5)88-9591
EDTAplasma(n=5)97-104101
heparinplasma(n=5)86-10194

Precision
Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighlevelTransformingGrowth转化生长因子β1(TGFβ1)检测试剂盒FactorBeta1(TGFb1)weretested20timesononeplate,respectively.
Inter-assayPrecision(Precisionbetweenassays):3sampleswithlow,middleandhighlevelTransformingGrowthFactorBeta1(TGFb1)weretestedon3differentplates,8replicatesineachplate.
CV(%)=SD/meanX100
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Linearity
ThelinearityofthekitwasassayedbytestingsamplesspikedwithappropriateconcentrationofTransformingGrowthFactorBeta1(TGFb1)andtheirserialdilutions.Theresultsweredemonstratedbythepercentageofcalculatedconcentrationtotheexpected.

Sample1:21:41:81:16
serum(n=5)81-103%82-105%80-91%97-104%
EDTAplasma(n=5)86-98%85-99%82-95%96-105%
heparinplasma(n=5)79-93%98-105%89-101%80-91%

Stability
Thestabilityofkitisdeterminedbythe转化生长因子β1(TGFβ1)检测试剂盒lossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.
Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperature首ldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.
Reagentsandmaterialsprovided

ReagentsQuantityReagentsQuantity
Pre-coated,readytouse96-wellstripplate1Platesealerfor96wells4
Standard2StandardDiluent1×20mL
DetectionReagentA1×120μLAssayDiluentA1×12mL
DetectionReagentB1×120μLAssayDiluentB1×12mL
TMBSubstrate1×9mLStopSolution1×6mL
WashBuffer(30×concentrate)1×20mLInstructionmanual1

Assayproceduresummary
1.Prepareallreagents,samplesandstandards;
2.Add100μLstandardorsampletoeachwell.Incubate2hoursat37oC;
3.Aspirateandadd100μL转化生长因子β1(TGFβ1)检测试剂盒preparedDetectionReagentA.Incubate1hourat37oC;
4.Aspirateandwash3times;
5.Add100μLpreparedDetectionReagentB.Incubate30minutesat37oC;
6.Aspirateandwash5times;
7.Add90μLSubstrateSolution.Incubate15-25minutesat37oC;
8.Add50μLStopSolution.Readat450nmimmediately.
Testprinciple
ThetestprincipleappliedinthiskitisSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoTransformingGrowthFactorBeta1(TGFb1).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecific转化生长因子β1(TGFβ1)检测试剂盒toTransformingGrowthFactorBeta1(TGFb1).Next,AvidinconjugatedtoHorseradishPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainTransformingGrowthFactorBeta1(TGFb1),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofTransformingGrowthFactorBeta1(TGFb1)in转化生长因子β1(TGFβ1)检测试剂盒thesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.

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2004-09-16