采用微量热法表征无共价键之核酸与小分子和蛋白质的结合反应

文档简介Theexpressionorreplicationofgenesisaffectedbythebindingofsmallmoleculeligandsandproteinstonucleicacidsequences.Suchbindingeventsarecriticalforthephysiologicalintegrityoforganismsandthereforeareoffundamentalinteresttolifescientists.Recently,thethermodynamicsdrivingtheseinteractionshavealsobecomeimportanttopharmaceuticalscientistsinvestigatingtheanticancer,antibacterialandantiviralpotentialofnucleicacid/ligandinteractions.Inaddition,asthenumberofdiseasesidentifiedasbeingduetoamalfunctionofcellularcontrolprocessesincreases,thepossibilityoftreatingdisordersbymanipulatinggeneexpressionisfurtherfocusingattentiononthethermodynamicsunderlyingnucleicacidbindingaffinityandspecificity.