讲座简介
外泌体是一类直径约30-150 nm的细胞外囊泡,可携带RNA,DNA,蛋白质、等多种信号分子,是一种新型的细胞间信号传递的媒介。在疾病的发生、诊断和治 疗中发挥着重要的作用。但是对于外泌体进行分析的手段却非常有限,尤其是在单个外泌体的表征的分析以外泌体内容物分析方面更加缺乏。美国NanoView Biosciences推出的全自动外泌体荧光检测分析系统—ExoView R200,采用了全新的SP-IRIS技术,实现了对单个外泌体的全面表征,包括了外泌体的荧光成像、亚群粒径检测、计数、内容物分析、蛋白共定位等。短短三年,在全世界范围内已有100多家实验室采用该设备,发表SCI近200篇,尤其在2022年使用Exoview在高水平期刊《Journal of Extracellular Vesicle》发表的文章已达到9篇!
2021年,Quantum Design中国子公司将Exoview R200引入中国,为国内科研工作者提供了多层次和全面的外泌体表征解决方案,同时也被生物通评为“2021生命科学十大创新产品”。短短两年时间,国内已经有多个单位使用全自动外泌体荧光检测分析系统发表高水平文章。
本次报告主要包括了:
盘点目前外泌体研究的主要方向、常规方法及其面临的限制
全面介绍单粒子干涉反射成像传感器(SP-IRIS)技术
展现Exoview检测外泌体内容物的优势
探讨外泌体亚群分析在外泌体研究的重要性
解析外泌体研究在血液检测、工业生产、载药递送、肿瘤诊断、阿尔茨海默病、脊髓受伤等领域的前沿进展
展示Exoview在《Journal of Extracellular Vesicle》、《Lab on a Chip》、《Nanoletters》等国际知名期刊的精选文章
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报告时间
开始 2023年6月27日 14:00
结束 2023年6月27日 14:30
主讲人介绍
李宁,药理学硕士,毕业于兰州大学。主要研究方向为生物制药研发和高通量检测。现任Quantum Design中国子公司产品经理,主要负责全自动外泌体荧光检测分析系统的应用开发、技术沟通及市场拓展的工作。
2022-2023年Exoview高水平文章精选列表:
Richard J. R. Kelwick …& Paul S. Freemont. (2023) Opportunities to accelerate extracellular vesicle research with cell-free synthetic biology. Journal of Extracellular Vesicles.
Laurence Blavier …& Yves A. DeClerck. (2023) The capture of extracellular vesicles endogenously released by xenotransplanted tumours induces an inflammatory reaction in the premetastatic niche. Journal of Extracellular Vesicles.
Hongyun Wang ……& Junjie Xiao. (2022) Extracellular vesicles enclosed-miR-421 suppresses air pollution (PM2.5)-induced cardiac dysfunction via ACE2 signalling. Journal of Extracellular Vesicles.
Linglei Jiang……& Santosh Dhakal. (2022) A7 bacterial extracellular vesicle-based intranasal vaccine against SARS-CoV-2 protects against disease and elicits neutralizing antibodies to wild-type and Delta variants. Journal of extracellular vesicles.
Shaobo Ruan, Nina Erwin, Mei He. (2022) Light-induced high-efficient cellular production of immune functional extracellular vesicles. Journal of extracellular vesicles.
Nasibeh Karimi ……& Cecilia Lässer. (2022) Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma – Contributions of platelet extracellular vesicles in plasma samples. Journal of Extracellular Vesicles.
Heikki Kyykallio ……& Pia R-M Siljander. (2022) A quick pipeline for the isolation of 3D cell culture-derived extracellular vesicles. Journal of Extracellular Vesicles.
Roberto Frigerio ……& Marina Cretich. (2022) Comparing digital detection platforms in high sensitivity immune-phenotyping of extracellular vesicles. Journal of Extracellular Vesicles.
Yael Hirschberg ……& Inge Mertens. (2022) Characterizing extracellular vesicles from individual low volume cerebrospinal fluid samples, isolated by SmartSEC. Journal of Extracellular Vesicles.
Sukhbir Kaur……& David D. Roberts. (2022) Single vesicle analysis of CD47 association with integrins and tetraspanins on extracellular vesicles released by T lymphoblast and prostate carcinoma cells. Journal of Extracellular Vesicles.
Simone M. Crivelli……& Erhard Bieberich. (2022) Function of ceramide transfer protein for biogenesis andsphingolipid composition of extracellular vesicles. Journal of Extracellular Vesicles.
Gi Beom Kim …& In-San Kim. (2023) Harnessing Oncolytic Extracellular Vesicles for Tumor Cell-Preferential Cytoplasmic Delivery of Misfolded Proteins for Cancer Immunotherapy. Small.
J. Klein, Y. Cao. (2023) Multi-Lipid Synergy In Cartilage Lubrication: Redundancy Or Evolution? Osteoarthritis and Cartilage.
Valerie DeLuca …& Michael E. Berens. (2023) Extracellular vesicles as a pharmacodynamic reporter in glioblastoma. Cancer Research.
Sarah Pike …& Liya Xu. (2023) Extracellular vesicles in diagnostic retinoblastoma aqueous humor correlate with disease stage and clinical outcome: a pilot study. Cancer Research.
D. Cookson …& Y. Ashraf Kharaz. (2023) The Biological Response Of Anterior Cruciate Ligamentocytes Following Treatment With Synovial Fluid Extracellular Vesicles. Osteoarthritis and Cartilage.
Mei Lu …& Yuanyu Huang. (2023) Antitumor synergism between PAK4 silencing and immunogenic phototherapy of engineered extracellular vesicles. Acta Pharmaceutica Sinica B.
Luke C. McIlvenna ……& Martin Whitham. (2023) Single vesicle analysis reveals the release of tetraspanin positive extracellular vesicles into circulation with high intensity intermittent exercise. The Journal of Physiology.
Joseph Blommer ……& Dimitrios Kapogiannis. (2023) Extracellular vesicle biomarkers for cognitive impairment in Parkinson’s disease. BRAIN.
Tyler J ……& Atta Behfar. (2022) Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence. Npj Regenerative Medicine.
Min Han ……& Tao Xin. (2022) Three-Dimensional-Cultured MSC-Derived Exosome-Hydrogel Hybrid Microneedle Array Patch for Spinal Cord Repair. Nano Letters.
Zijian Yang ……& David A. Issadore. (2022) Ultrasensitive Single Extracellular Vesicle Detection Using High Throughput Droplet Digital Enzyme-Linked Immunosorbent Assay. Nano Letters.
中国客户发表文章精选:
上海大学肖俊杰课题组在《Journal of extracellular vesicles》发表文章,题目为《Extracellular vesiclesenclosed-miR-421 suppresses air pollution (PM2.5)-induced cardiacdysfunction via ACE2 signalling》
中国科学院深圳技术研究院杨慧课题组发表在《Lab on a Chip》发表文章,题目为《Extraction of small extracellular vesicles by label-free and biocompatibleon-chip magnetic separation》
北京天坛医院张力伟课题组、国家纳米科学中心梁兴杰课题组、北京航空航天大学陈军歌课题组在《Advanced Science》发表文章,题目为《Functionalized Macrophage Exosomeswith Panobinostat and PPM1D-siRNA for Diffuse Intrinsic Pontine Gliomas Therapy》。
同济大学附属上海市肺科医院姜格宁/徐建芳/郑迪课题组、上海思路迪转化医学在《Journal of Nanobiotechnology》发表文章,题目为《Identificationand evaluation of circulating small extracellular vesicle microRNAs asdiagnostic biomarkers for patients with indeterminate pulmonary nodules》。
山东第 一医科大学辛涛/山东大学刘宏课题组在《NANO LETTERS》发表文章,题目为《Three-Dimensional-Cultured MSC-Derived Exosome-Hydrogel Hybrid Microneedle Array Patch for Spinal Cord Repair》
南京大学在李靓/张辰宇/张玉婧课题组《Frontiers in Cell and Developmental Biology》发表文章,题目为《Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo》
全自动外泌体荧光检测分析系统(ExoView R200)简介
Nanoview所开发的全自动外泌体荧光检测分析系统(ExoView R200)采用单粒子干涉反射成像传感器(SP-IRIS)技术,是一款无需纯化的全自动的新型外泌体表征设备。该设备能够提供全方位的外泌体表征信息,包括颗粒大小、计数、表型与生物标志物共定位等,提供多层次和全面的外泌体测量解决方案。
为了更好地服务中国客户;Quantum Design中国子公司在北京建立了专业的客户服务中心,正式推出专业的全方位外泌体表征测试服务,您只需要少量样品即可获得全方位的外泌体表征数据:
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