[ACE UltraCore SuperC18色谱柱]人参皂

[ACE UltraCore SuperC18色谱柱]人参皂甙分析

Conditions
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 100 x 3.0 mm; 2 x 100 x 3.0 mm (coupled); 3 x 100 x 3.0 mm (coupled)
Part Number: CORE-25A-1003U
Mobile Phase:

A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN

Gradient:

Time (mins)
100x3.0mm	200x3.0mm	300x3.0mm	%B
-	0.00	0.00	5
0.00	0.36	0.71	5
10.00	20.36	30.71	70
11.00	22.36	33.71	95
13.00	26.36	39.71	95
14.00	28.36	42.71	5
22.00	44.36	66.71	5

Flow Rate: 0.8 mL/min
Injection: 2 μL (100 x 3.0 mm); 4 μL (200 x 3.0 mm); 6 μL (300 x 3.0 mm)
Temperature: 80 °C
Detection: UV, 203 nm
Sample: 5 x 75 mg tablets ground to fine powder and extracted with 10.0 mL MeCN/H2O (1:1 v/v) for 15 minutes with ultrasonication. 100 μL supernatant diluted with 300 μL water and filtered using a Whatman Mini-Uniprep syringeless filter
System: Chromaster Ultra Rs