本研究利用胃蛋白酶酶解ZG毛虾制备ACEYZ产物,采用凝胶色谱法对酶解产物进行分离纯化,测定其分子量分布,确定高ACEYZ活性组分的分子量范围,并利用GX液相色谱进行纯化,采用M... 本研究利用胃蛋白酶酶解ZG毛虾制备ACEYZ产物,采用凝胶色谱法对酶解产物进行分离纯化,测定其分子量分布,确定高ACEYZ活性组分的分子量范围,并利用GX液相色谱进行纯化,采用MS/MS进行结构鉴定。
本研究利用胃蛋白酶酶解ZG毛虾制备ACEYZ产物,采用凝胶色谱法对酶解产物进行分离纯化,测定其分子量分布,确定高ACEYZ活性组分的分子量范围,并利用GX液相色谱进行纯化,采用MS/MS进行结构鉴定。:This research prepares ACE using the pepsin enzymolysis China shrimp to suppress the product, uses the gelatin chromatography to carry on the separation purification to the enzymolysis product, determines its molecular weight distribution, determined that high ACE suppresses the active constituent the molecular weight scope, and carries on the purification using the highly effective liquid chromatography, uses MS/MS to carry on the structure appraisal.