摘要：该文探讨了经45摄氏度热水提取及微波辅助热水提取，savage法去蛋白，浓缩，醇析，洗涤，干燥等步骤提取荸荠多糖，对比了常规提取和微波辅助热水提取的产率，并应用苯酚—硫酸法... 摘要：该文探讨了经45摄氏度热水提取及微波辅助热水提取，savage法去蛋白，浓缩，醇析，洗涤，干燥等步骤提取荸荠多糖，对比了常规提取和微波辅助热水提取的产率，并应用苯酚—硫酸法测定其含量，本法选取488nm为反应产物的测定波长，所测葡萄糖标准曲线相关系数r=0.9997，在20~100ug/mL范围内吸光度与被测物含量呈良好的线性关系，测得多糖的平均量为4.7914%，本法为反应物在160min内吸光度值稳定，重现性较好，平均回收率为96.14%，结果令人满意。
关键词：荸荠、多糖、微波萃取、苯酚—硫酸法、产率、提取、测定译成英文，谢谢！
a hot water of Celsiuses and the microwave assistance hot water withdraw， the savage method goes to the egg white， concentrated， the ， wash away dirt， dry etc. step withdraws many sugar of water chestnut， contrasting the normal regulations to withdraw and the microwave lend support to the hot water to withdraw of produce the rate， and apply the benzene - sulfuric acid method to measurese its content， this method selects by examinations the 488 nms for the measurement wave-length that respond the outcome， related coefficient r of standard curve of the glucose measure=0.9997， absorb the intensity of light and is measure within the scope of 20~100 ugs/ mL the thing content presents the good line sexual behavior， measure many sugar of average measure to 4.7914%， this method for respond the thing in the 160 mins absorb the intensity of light value stability， re-appear sex better， the average recovery rate is 96.14%， as a result giving satisfaction.
评论
1472581101 
长按识别二维码查看信息详情