片段翻译成中文

The amplification was carried out on a PTC-200 thermocycler (MJ Research) in a 30m l reaction mixture containing10mM Tris–HCl (pH 8.3)， 1.5mM MgCl2， 50mM KCl，150mM of each dNTP， 0.3mM of each primer (synthesized by TaKaRa)， 1.0m l (about 1... The amplification was carried out on a PTC-200 thermocycler (MJ Research) in a 30m l reaction mixture containing10mM Tris–HCl (pH 8.3)， 1.5mM MgCl2， 50mM KCl，150mM of each dNTP， 0.3mM of each primer (synthesized by TaKaRa)， 1.0m l (about 100 ng) template DNA， and 1U Taq DNA polymerase (Promega). The reaction mixtures were denatured at 95 °C for 5 min and subjected to 30 cycles of 40 s at 95 °C， 1 min at 55 °C， 1.5 min at 72 °C， and a final extension step of 7 min at 72 °C. Amplified products were purified using WizardTM PCR Prep DNA Kit (Promega) according to the manufacturer’s instructions， then the two strands were sequenced directly with PRISMTM BigDye Terminator Ready Reaction Kit (Applied Biosystems) on an ABI 310 Genetic Analyzer (Perkin Elmer).