求翻译，不要机器翻

All cell lines were grown in RPMI-1640 medium (GIBCO) supplemented with 10% heat-inactivated bovine serum， 2 nM L-glutamine， 105 IU/liter penicillin G， 100 mg/liter streptomycin and 10 mM HEPES， pH 7.4. Cells were kept at 37°C in a humidif... All cell lines were grown in RPMI-1640 medium (GIBCO) supplemented with 10% heat-inactivated bovine serum， 2 nM L-glutamine， 105 IU/liter penicillin G， 100 mg/liter streptomycin and 10 mM HEPES， pH 7.4. Cells were kept at 37°C in a humidified 5% CO2 incubator.