毛细管电色谱（capillary electrochromatography，CEC）是在毛细管电泳技术不断发展和GX液相色谱理论日益完善的基础上发展起来的一种具有两者双重分离性能的分离分析方法[64，65]。它在毛细管内填充液相色谱固定相或在管壁键合固定相，在两端施加高电压，以... 毛细管电色谱（capillary electrochromatography，CEC）是在毛细管电泳技术不断发展和GX液相色谱理论日益完善的基础上发展起来的一种具有两者双重分离性能的分离分析方法[64，65]。它在毛细管内填充液相色谱固定相或在管壁键合固定相，在两端施加高电压，以电渗流驱动流动相经过色谱分离柱，溶质根据它们在固定相和流动相之间的分配以及它们电泳淌度的不同而得以分离。CEC以具有塞子流型的电渗流代替了具有抛物线流型的压力流，因而具有毛细管区带电泳(CZE) 的高柱效。CEC 采用液相色谱的固定相和流动相， 因而还具有GX液相色谱的高选择性。因此，CEC 自诞生以来就一直是分析化学研究领域的热点课题，并已逐渐进入应用领域[66，67]。
CEC 的核心部件是毛细管电色谱柱。根据固定相存在形式的不同，毛细管
电色谱柱被分为开管柱、填充柱和整体柱。运行中的焦耳热效应、塞子界面效应和气泡效应限制了填充毛细管电色谱（packed-column CEC，PCCEC）柱的发展和应用[68，69]。整体柱则存在溶胀、受热变形、机械性能差等不足，并且很难获得孔径分布非常均一的连续床，因此分离小分子化合物时很难得到较高的柱效[2]。开管柱制备和运行过程简单、易于控制，柱效高，且OTCEC 中的电渗流流速比PCCEC 中的大60% [70]，因此更适于快速分析。但是，其Z大的缺点是相比小，柱容量低。因此制备开管柱的关键是增加表面积，提高相比和柱容量。
本节中，我们将上节制备的十八烷基改性纳米二氧化硅涂层毛细管柱应用于
OTCEC 领域，希望通过液相沉积的纳米颗粒来增加固定相的相比，提高柱容量。
随后，我们研究了该柱的电渗流行为，并对电色谱性能进行了评价。
CEC (capillary electrochromatography， CEC) is the continuous development of capillary electrophoresis and high performance liquid chromatography increasingly sophisticated theories developed on the basis of a double separation performance between the two analytical methods of separation [64，65] . It filled in a capillary liquid chromatography stationary phase or in the wall bonded stationary phase， high-voltage imposed at both ends to electroosmotic flow-driven mobile phase through column chromatography， solute according to their fixed and mobile phase between the phase distribution as well as their different electrophoretic mobility and can be separated. CEC in a plug flow pattern instead of the electroosmotic flow has a parabolic flow pattern of the pressure flow， which has a capillary zone electrophoresis (CZE) high column efficiency. CEC liquid chromatography using the stationary phase and mobile phase， which also has a high selectivity of high-performance liquid chromatography. Therefore， CEC has been since its birth in Analytical Chemistry is a hot area of research topics and application areas have gradually [66，67].
CEC is the core component of capillary electrochromatography column. Stationary phases based on the existence of different forms of capillary
Power was divided into open tubular column， packed column and monolithic column. In the operation of the Joule heating effect， interface effect plug and fill the bubble effect of limiting the CEC (packed-column CEC， PCCEC) the development and application of column [68，69]. Monolithic column there is swelling， heat deformation， insufficient and poor mechanical properties， and it is difficult to obtain a very uniform pore size distribution for bed， so when the separation of small molecule compounds difficult to obtain higher column efficiency [2]. Preparation of open tubular column and run the process as simple and easy to control， high column efficiency and OTCEC the electroosmotic flow velocity ratio PCCEC large 60% [70] and therefore are more suitable for rapid analysis. However， its biggest drawback is that compared to small， low-capacity columns. Preparation of open tubular column is therefore the key is to increase the surface area， improve the capacity compared to column.
This section， we will be prepared in the previous section octadecyl modified nano-silica coated capillary column used
OTCEC the field， and hopes that the nano-particle liquid phase deposition to increase compared to the stationary phase to enhance the capacity of column.
Subsequently， we looked at the column of the electroosmotic flow behavior， and the performance of electrochromatography is evaluated.
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