1、DNA样品的纯度与哪些因素有关?2、从外周血提取DNA尽量用新鲜血,为什么?3、长期保存DNA样品时其稳定性受哪些因素影响?请达人指点,谢谢啦。。。好的追加分!!!... 1、DNA样品的纯度与哪些因素有关?
2、从外周血提取DNA尽量用新鲜血,为什么?
3、长期保存DNA样品时其稳定性受哪些因素影响?
请达人指点,谢谢啦。。。
好的追加分!!!
1. impurity of a DNA preparation is proteins or/and RNA. So, you would like to have DAN preparation with high quality and purity, you have to get rid of proteins and RNA.
2. fresh blood would make good quality of DNA. If you keep blood for long time, because it is so nutrition-rich material, bacteria may grow, that will ruin your samples. Also, DNA source in blood is from white blood cells. You would not like white cell autolysis which will lead DNA degradation, you would like to prepare DNA from fresh samples.
3. DNA should be kept at -80 centigrade degree for long storage, or -20 degree, or 4 degree for short term storage, and it should be in TE buffer. pH8.0 is good for DNA preservation. Try not use water. Therefore, low temperature, pH value around 8.0, and chelate (EDTA) are factors necessary for DNA storage.