Synthesisofsquarainedyeswasreportedatleastin1966。Theyarederivedfromsquaricacidwhichundergoesanelectrophilicaromaticsubstitutionreactionwithananilineoranotherelectronrich... Synthesis of squaraine dyes was reported at least in 1966 。They are derived from squaric acid which undergoes an electrophilic aromatic substitution reaction with an aniline or another electron rich derivative to form a highly conjugated product with extensive charge distribution. For instance, squaraine dyes are also formed via reaction of squaric acid or its derivatives with so-called "methylene bases" like 2-methyl-indolenines, 2-metyl-benzthiazoles or 2-methyl-benzo-selenazoles. Indolenine-based squaraines combine good photostability including high quantum yields when bound to proteins and reactive versions of these dyes are commonly used as fluorescent probes and labels for biomedical applications .
报道了合成染料的squaraine至少在1966年,他们是来自squaric酸,electrophilic芳香取代反应经历了一段与苯胺衍生物或另一个电子丰富,形成一个高度的共轭产品有广泛的电荷分布。例如,squaraine染料的squaric通过反应形成酸或其衍生物和所谓的“甲基地”,2-metyl-benzthiazoles或2-methyl-benzo-selenazoles像2-methyl-indolenines。Indolenine-based联合好squaraines光化学稳定性包括高蛋白质产量的时候一定要量子版本的这些染料及无功补偿装置通常被用做荧光探针和标签生物技术上的应用。