仪器资讯
白介素29(IL29)检测试剂盒
适用生物 Homo sapiens (Human，人)
白介素29(IL29)检测试剂盒检测范围 7.81-500pg/mL 灵敏度 2.9pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法 白介素29(IL29)检测试剂盒规格 96T
ELISA Kit for Interleukin 29 (IL29)
白介素29(IL29)检测试剂盒FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism speciesHomo sapiens (Human)Product No.SEC029HuSample typeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Format96-well strip plateAssay length4.5 hoursDetection range7.81-500pg/mL The standard curve concentrations used for the ELISA’s were 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL, 7.81pg/mLSensitivityThe minimum detectable dose of this kit is typically less than 2.9pg/mL.白介素29(IL29)检测试剂盒Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 29 (IL29).
No significant cross-reactivity or interference between Interleukin 29 (IL29) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 29 (IL29) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 29 (IL29) in samples.
MatrixRecovery range (%)Average(%)serum(n=5)99-105102EDTA plasma(n=5)97-105101heparin plasma(n=5)94-104101Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 29 (IL29) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 29 (IL29) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
白介素29(IL29)检测试剂盒Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 29 (IL29) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:81:16serum(n=5)89-97%83-92%80-96%87-101%EDTA plasma(n=5)92-101%97-105%96-104%92-101%heparin plasma(n=5)87-94%79-101%95-105%80-101%Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature 首ld be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
ReagentsQuantityReagentsQuantityPre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4Standard2Standard Diluent1×20mLDetection Reagent A1×120μLAssay Diluent A1×12mLDetection Reagent B1×120μLAssay Diluent B1×12mLTMB Substrate1×9mLStop Solution1×6mLWash Buffer (30 × concentrate)1×20mLInstruction manual1白介素29(IL29)检测试剂盒Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50μL Stop Solution. Read at 450nm immediately.
白介素29(IL29)检测试剂盒Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 29 (IL29). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 29 (IL29). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 29 (IL29), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 29 (IL29) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
[ 打印 ] [ 返回顶部 ] [ 关闭 ]
