仪器资讯
22Rv1
GeneralInformation:
Organism:
Homosapiens,human
Tissue:
prostate
CultureProperties:
adherent
Morphology:
epithelial
CultureMethod:
CompleteGrowthMedium:
RPMI-1640(Gibco31800-022)+10%FBS+Penicillin/Streptomycin
Subculturing:
Volumesaregivenfora25cm2flask.Increaseordecreasetheamountofdissociationmediumneededproportionallyforculturevesselsofothersizes.
Removeanddiscardculturemedium.Brieflyrinsethecelllayerwith0.25%(w/v)Trypsin-EDTAsolutiontoremovealltracesofserumthatcontainstrypsininhibitor.Add1.0to2.0mLofTrypsin-EDTAsolutiontoflaskandobservecellsunderaninvertedmicroscopeuntilcelllayerisdispersed.DiscardTrypsin-EDTAsolution.Add6.0to8.0mLofcompletegrowthmediumandaspiratecellsbygentlypipetting.Addappropriatealiquotsofthecellsuspensiontonewculturevessels.Incubateculturesat37°C,5%CO2.SubcultivationRatio:
Asubcultivationratioof1:3to1:6isrecommended
Cryopreservation:
Freezemedium:Completegrowthmediumsupplementedwith10%(v/v)DMSO
Storagetemperature:liquidnitrogenvaporphase
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