elisa试剂盒样本处理及要求:
1.血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。
2.血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。
3.尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。
4.细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5.组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
6.标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.
elisa试剂盒Human15-lipoxygenase,15-LO/LOXELISAKit人15脂加氧酶(15-LO/LOX)ELISA试剂盒规格:96T/48T
Human16-αHydroxyestrogen1,16-αOHE-1ELISAKit人16α羟基雌酮1(16-αOHE-1)ELISA试剂盒规格:96T/48T
Human17-Hydroxycorticosteroids,17-OHCSELISAKit人17羟皮质类固醇(17-OHCS)ELISA试剂盒规格:96T/48T
Human17-Hydroxyprogesterone,17-OHPELISAKit人17羟孕酮(17-OHP)ELISA试剂盒规格:96T/48T
Human17-ketosteroids,17-KSELISAKit人17-酮类固醇(17-KS)ELISA试剂盒规格:96T/48T
Human2,3-dinorthromboxaneB2,2,3-dinor-TXB2ELISAKit人2,3Dinor血栓烷B2(2,3-dinor-TXB2)ELISA试剂盒规格:96T/48T
Human2,3-Disphosphoglycerate,2,3-DPGELISAKit人2,3-二磷酸甘油酸(2,3-DPG)ELISA试剂盒规格:96T/48T
Human20-hydroxyeicosatetraenoicacid,12-HETEELISAKit人12羟二十烷四烯酸(12-HETE)ELISA试剂盒规格:96T/48T
Human25-DihydroxyvitaminD3,HVD3ELISAKit人25羟基维生素D3(25(OH)D3/25HVD3)ELISA试剂盒规格:96T/48T
Human26Sproteasome,26SPSMELISAKit人26S蛋白酶体(26SPSM)ELISA试剂盒规格:96T/48T
Human28SribosomeRNPantibody,28SrRNPELISAKit人28S抗核糖体抗体(28SrRNP)ELISA试剂盒规格:96T/48T
human3-hydroxy-3-methylglutaryl-CoenzymeAsynthase1,HMGCS1ELISAKit人3-羟基-3-甲基戊二酰辅酶A合酶1(HMGCS1)ELISA试剂盒规格:96T/48T
Human3-Nitrotyrosine,3-NTELISAKit人3-硝基酪氨酸(3-NT)ELISA试剂盒规格:96T/48T
Human50%complementhemolysis,CH50ELISAKit人血清总补体(CH50)ELISA试剂盒规格:96T/48T
Human5-hydroxyindolaceticacid,5-HIAAELISAKit人5羟基吲哚乙酸(5-HIAA)ELISA试剂盒规格:96T/48T
Human5-Hydroxytryptamine,5HTELISAKit人5羟色胺(5-HT)ELISA试剂盒规格:96T/48T
Human5-lipoxygenase,5-LO/LOXELISAKit人5脂加氧酶(5-LO/LOX)ELISA试剂盒规格:96T/48T
Human5-Nucleotidase,5-NTELISAKit人5核苷酸酶(5-NT)ELISA试剂盒规格:96T/48T
Human6-hydroxydopamine,6-OHDAELISAKit人6-羟多巴胺(6-OHDA)ELISA试剂盒规格:96T/48T
Human6-keto-prostaglandinF1a,6-K-PGF1aELISAKit人6酮前列腺素F1a(6-keto-PGF1a)ELISA试剂盒规格:96T/48T
Human6-keto-prostaglandin,6-K-PGELISAKit人6酮前列腺素(6-K-PG)ELISA试剂盒规格:96T/48T
Human8-epi-prostaglandinF2alpha,8-epi-PGF2αELISAKit人8-异构前列腺素F2α(8-epi-PGF2α)ELISA试剂盒规格:96T/48T
Human8-isoprostaglandin,8-iso-PGELISAKit人8异前列腺素(8-iso-PG)ELISA试剂盒规格:96T/48T
HumanADisintegrinAndMetalloprotease10,ADAM10ELISAKit人解整合素样金属蛋白酶10(ADAM10)ELISA试剂盒规格:96T/48T
HumanADisintegrinAndMetalloprotease8,ADAM8ELISAKit人解整合素样金属蛋白酶8(ADAM8)ELISA试剂盒规格:96T/48T
HumanADisintegrinAndMetalloprotease9,ADAM9ELISAKit人解整合素样金属蛋白酶9(ADAM9)ELISA试剂盒规格:96T/48T
HumanAbnormalprothrombin,APTELISAKit人异常凝血酶原(APT)ELISA试剂盒规格:96T/48T
Humanacetylcholinereceptorantibody,AChRabELISAKit人乙酰胆碱受体抗体(AChRab)ELISA试剂盒规格:96T/48T
Humanacetylcholine,ACHELISAKit人乙酰胆碱(ACH)ELISA试剂盒规格:96T/48T
HumanAcetylcholinesterase,AChEELISAKit人乙酰胆碱酯酶(AChE)ELISA试剂盒规格:96T/48T
HumanAcidPhosphatase,ACPELISAKit人酸性磷酸酶(ACP)ELISA试剂盒规格:96T/48T
Humanacidsphingomyelinase,ASMELISAKit人酸性神经鞘磷脂酶(ASM)ELISA试剂盒规格:96T/48T
Humanacidicfibroblastgrowthfactor1,aFGF-1ELISAKit人酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒规格:96T/48T
Humanaconitase2,ACO-2ELISAKit人乌头酸酶2(ACO-2)ELISA试剂盒规格:96T/48T
HumanAcrineOrange,AOELISAKit人吖啶橙(AO)ELISA试剂盒规格:96T/48T
HumanactivatedcoagulationfactorⅫ,FⅫaELISAKit人活化凝血因子Ⅻ(FⅫa)ELISA试剂盒规格:96T/48T
HumanActivatedLeukocyteCellAdhesionMolecule,ALCAMELISAKit人白细胞活化黏附因子(ALCAM)ELISA试剂盒规格:96T/48T
HumanactivatedproteinCresistance,APCRELISAKit人活化蛋白C抵抗素(APCR)ELISA试剂盒规格:96T/48T
HumanactivatedproteinC,APCELISAKit人活化蛋白C(APC)ELISA试剂盒规格:96T/48T
Humanactivatingtranscriptionfactor-1,ATF-1ELISAKit人转录因子抗体-1(ATF-1)ELISA试剂盒规格:96T/48T
HumanActivinA,ACV-AELISAKit人活化素A(ACV-A)ELISA试剂盒规格:96T/48T
HumanAcylationStimulatingProtein,ASPELISAKit人酰化刺激蛋白(ASP)ELISA试剂盒规格:96T/48T
humanADAMmetallopeptidasewiththrombospondintype1motif4,ADAMTS4ELISAKit人ADAM金属肽酶含血小板反应蛋白1基元4(ADAMTS4)ELISA试剂盒规格:96T/48T
Humanadaptormoleculeapoptoticpeptidaseactivatingfactor1,APAF1ELISAKit人衔接蛋白酶活化因子1(APAF1)ELISA试剂盒规格:96T/48T
Humanadeno-associatedvirus,AAVELISAKit人腺相关病毒(AAV)ELISA试剂盒规格:96T/48T
Humanadenosinedeaminase,ADAELISAKit人腺苷脱氨酶(ADA)ELISA试剂盒规格:96T/48T
HumanAdenosinediphosphate,ADPELISAKit人二磷酸腺苷(ADP)ELISA试剂盒规格:96T/48T
HumanAdenovirusAntigen,ADV-AgELISAKit人腺病毒抗原(ADV-Ag)ELISA试剂盒规格:96T/48T
HumanAdenovirusIgG,ADV-IgGELISAKit人腺病毒IgG(ADV-IgG)ELISA试剂盒规格:96T/48T
HumanAdenovirusIgM,ADV-IgMELISAKit人腺病毒IgM(ADV-IgM)ELISA试剂盒规格:96T/48T
Humanadenylylcyclase1,AC-1ELISAKit人腺苷酸环化酶1(AC-1)ELISA试剂盒规格:96T/48T
Humanadipocytefattyacid-bindingprotein,a-FABPELISAKit人脂肪细胞型脂肪酸结合蛋白(aFABP)ELISA试剂盒规格:96T/48T
Humanadiponectin,ADPELISAKit人脂联素(ADP)ELISA试剂盒规格:96T/48T
Humanadiposedifferentiation-relatedprotein,ADRPELISAKit人脂肪分化相关蛋白(ADRP)ELISA试剂盒规格:96T/48T
Humanadrenalcortexantibody,ACAELISAKit人肾上腺皮质抗体(ACA)ELISA试剂盒规格:96T/48T
humanadrencocorticotropichormone,ACTHELISAKit人促肾上腺皮质激素(ACTH)ELISA试剂盒规格:96T/48T
Humanadrenergicalpha-1Areceptor,ADRA1AELISAKit人肾上腺素能a1A受体(ADRA1A)ELISA试剂盒规格:96T/48T
Humanadrenomedullin,ADMELISAKit人肾上腺髓质素(ADM)ELISA试剂盒规格:96T/48T
Humanadvancedglycationendproducts,AGEsELISAKit人晚期糖基化终末产物(AGEs)ELISA试剂盒规格:96T/48T
Humanadvancedoxidationproteinproducts,AOPPELISAKit人晚期氧化蛋白产物(AOPP)ELISA试剂盒规格:96T/48T
HumanAgoutiRelatedProtein,AGRPELISAKit人Agouti相关蛋白(AGRP)ELISA试剂盒规格:96T/48T
HumanAgrinELISAKit人聚集蛋白(Agrin)ELISA试剂盒规格:96T/48T
HumanAlanineAminotransferase,ALT/GPTELISAKit人丙氨酸转氨酶/谷丙转氨酶(ALT/GPT)ELISA试剂盒规格:96T/48T
Humanalcoholdehydrogenase,ADHELISAKit人乙醇脱氢酶(ADH)ELISA试剂盒规格:96T/48T
Humanaldehydedehydrogenase,ALDHELISAKit人乙醛脱氢酶(ALDH)ELISA试剂盒规格:96T/48T
HumanAldolase,ALDELISAKit人醛缩酶(ALD)ELISA试剂盒规格:96T/48T
humanaldosterone,ALDELISAKit人醛固酮(ALD)ELISA试剂盒规格:96T/48T
Humanalkalinephosphatase,ALPELISAKit人碱性磷酸酶(ALP)ELISA试剂盒规格:96T/48T
HumanAlpha1Antichymotrypsin,AACTELISAKit人α1抗胰糜蛋白酶(AACT)ELISA试剂盒规格:96T/48T
Humanalpha-1-microglobulin/bikuninprecursor,AMBPELISAKit人α1微球蛋白/bikunin前体(AMBP)ELISA试剂盒规格:96T/48T
HumanAlpha-Actin,αActinELISAKit人α肌动蛋白(αActin)ELISA试剂盒规格:96T/48T
Humanalpha-fetoproteinLensculinarisagglutiin1,AFP-L1ELISAKit人小扁豆素结合型甲胎蛋白/甲胎蛋白异质体1(AFP-L1)ELISA试剂盒规格:96T/48Telisa试剂盒
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