采用相干反斯托克斯拉满散射和其他非线性光学机制进行光

In this work multimodal operation of commercially available CARS
microspectrometer (EKSPLA CARSCOPE) equipped with a picosecond
dual wavelength laser source (EKSPLA PT259) was demonstrated for
the imaging of various biological objects using coherent anti‑Stokes
Raman scattering (CARS), two photon excited fluorescence (TPEF)
and second harmonic generation (SHG) contrast mechanisms.
Obtained CARS‑images of yeast cell and flower pollen clearly
demonstrate label‑free and 3D imaging capabilities of CARS technique.
Multi‑contrast images of green algae and plants containing starch
demonstrate a variety of research facilities, where the method can be
applied. Ekspla CARS 相干反斯托克斯拉曼显微光谱仪 PL2230系列高能量全固态锁模皮秒Nd:YAG激光器