Sar1-GTP 小鼠单抗/NeweastBio现货-武汉费斯德生物科技有限公司

详细信息

货号: 26916

规格: 30 µL

基因符号: SAR1

描述: Anti-Sar1-GTP Mouse Monoclonal Antibody

背景介绍: Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER.

免疫原: Recombinant full length protein of active Sar1a.

经过测试的应用: IP, IHC, IF

推荐稀释比:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250

浓度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亚型: IgG

纯度: Purified from ascites

防腐剂: no

成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

种属反应性: Anti-active Sar1 antibody recognizes active Sar1 of vertebrates.

储存条件: Store at -20°C. Avoid repeated freezing and thawing

免疫沉淀/蛋白质印迹:

Sar1 Activation Assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No. 26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody (Cat. No. 21090).

主要用途

GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP，因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性，可将GTP水解为GDP，从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。