Anti-RhoA-GTP Mouse Monoclonal Antibody-武汉费斯德生物科技有限公司

详细信息

货号: 26904

规格: 30 µL

基因符号: RHOA

描述: Anti-RhoA-GTP Mouse Monoclonal Antibody

背景介绍: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction.

免疫原: Recombinant full length protein of active RhoA

经过测试的应用: IP, IHC,IF

推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250

浓度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亚型: IgG2b

纯度: Purified from ascites

防腐剂: No

成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol

种属反应性: Anti-RhoA monoclonal antibody recognizes active RhoA from vertebrates.

储存条件: Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation/Western Blot:

IP/WB Analysis of Active RhoA Protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-RhoA-GTP monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26007).

主要用途

GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP，因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性，可将GTP水解为GDP，从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。