pSV2-dhfr

质粒类型：哺乳动物表达载体

启动子：SV40

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：4900 bp 

5' 测序引物及序列：SV40pro-F:TATTTATGCAGAGGCCGAGG

3' 测序引物及序列：SV40pA-R:GAAATTTGTGATGCTATTGC

载体标签：C-DHFR ( mouse DHFR)

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：MTX (甲氨蝶呤)

备注：pSV2-DHFR载体含有的是小鼠的DHFR基因，同时该载体是哺乳动物细胞和大肠杆菌穿梭质粒，在DHFR细胞系中，可以通过甲氨蝶呤对转染的细胞进行筛选。

A mammalian cell/E.coli shuttle vector. Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture strains. (ATCC staff) Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--5.2; PvuII/HindIII--4.8, 0.3; BglII/EcoRI--3.6, 1.5; AccI/HindIII--3.6, 0.54, 0.44, 0.27; AccI--3.6, 0.96, 0.27; HindIII--5.0; PstI--4.1, 0.9. (ATCC staff) Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; HindIII--4.8; BamHI--4.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli MC1061, vertebrate cells, E.coli.