调制叶绿素荧光成像系统——IMAGING-PAM

主要功能

• 成像功能：对 Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 种参数进行成像分析。测定调节性能量耗散 Y(NPQ)，反映植物光保护能力，测定非调节性能量耗散Y(NO)，反映植物光损伤程度。

• 程序测量功能：可程序测量荧光诱导曲线、快速光曲线和暗弛豫，也可手动测量；在测量过程中能自动分析所有荧光参数的变化趋势

• AOI 功能：可在测量前或测量后任意选择感兴趣的区域（AOI），程序将自动对选择的 AOI 的数据进行变化趋势分析，并在报告文件中显示相关 AOI 的数据。所有报告文件中显示的数据都可导出到 EXCEL 文件中。

• 成像异质性分析功能：对任意参数任意时间的成像，可在图像上任意选取两点，软件自动对两点间的数据进行横向异质性分析，并可导出到 EXCEL 文件中。

• 成像数据范围分析功能：对任意参数任意时间的成像，可分析任意两个荧光数值之间有多少个像素点，多少面积（cm²）。

• 突变株筛选功能：可跟据成像结果快速筛选光合、产氢/油、抗逆（抗盐、抗旱、抗病等）等突变株。

• 微藻毒理研究功能：可同时测量 96 个微藻样品（对照和处理组）的光合活性，软件自动给出处理组样品相对于对照组的光合YZ百分比。

• 吸光系数测量功能：快速测量叶片的吸光系数。吸光系数测量光源： 16 个红光（650 nm）和 16 个近红外（780 nm）LED，用于测量植物叶片或藻类样品 PAR 吸光系数。

测量参数

Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

应用领域

• 光合作用研究：可以在完全相同的条件下同时对大量样品进行成像

• 植物病理学：病斑部位（包括肉眼不可见时）成像以及病斑扩散的时空动力学

• 植物胁迫生 理学：肉眼不可见胁迫损伤的早期检测

• 遗传育种：出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株

• 突变株筛选：快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等

• 微藻毒理学：不同毒物浓度多个重复的样品一次测完，软件自动计算YZ比率

• 分子生物学：宏观水平上检测样品的绿色荧光蛋白（GFP）荧光

• 其它多种扩展研究

  
  

选购指南

一、荧光成像 MAXI 版

系统组成：通用型主机，LED 供电单元， LED 光源，CCD，数据线，软件等。

注意：高等植物或真核藻类测量时选择蓝光光源（推荐），蓝藻测量请选择红光光源。

荧光成像 MAXI 版本

二、荧光成像 MINI 版

系统组成：通用型主机（下图中未显示），LED 光源（如下图），CCD，数据线，软件等。

注意：高等植物或真核藻类测量时选择蓝光光源（推荐），蓝藻测量请选择红光光源。

荧光成像 MINI 版

三、荧光成像 MICROSCOPY 版

系统组成：通用型主机，特制显微镜，CCD，数据线，软件等。

荧光成像 MICROSCOPY 版

葡萄叶片 Y(NPQ) 成像	葡萄叶片 PS/50 成像结果	MINI 版本成像结果
显微版本成像结果	软件成像界面	慢速诱导动力学曲线

产地：德国 WALZ

数据来源：光合作用文献 Endnote 数据库，更新至 2021 年 1 月，文献数量超过 10000 篇

原始数据来源：Google Scholar

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