调制叶绿素荧光成像系统——IMAGING-PAM

主要功能

• 成像功能：对 Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 种参数进行成像分析。测定调节性能量耗散 Y(NPQ)，反映植物光保护能力，测定非调节性能量耗散Y(NO)，反映植物光损伤程度。

• 程序测量功能：可程序测量荧光诱导曲线、快速光曲线和暗弛豫，也可手动测量；在测量过程中能自动分析所有荧光参数的变化趋势

• AOI 功能：可在测量前或测量后任意选择感兴趣的区域（AOI），程序将自动对选择的 AOI 的数据进行变化趋势分析，并在报告文件中显示相关 AOI 的数据。所有报告文件中显示的数据都可导出到 EXCEL 文件中。

• 成像异质性分析功能：对任意参数任意时间的成像，可在图像上任意选取两点，软件自动对两点间的数据进行横向异质性分析，并可导出到 EXCEL 文件中。

• 成像数据范围分析功能：对任意参数任意时间的成像，可分析任意两个荧光数值之间有多少个像素点，多少面积（cm²）。

• 突变株筛选功能：可跟据成像结果快速筛选光合、产氢/油、抗逆（抗盐、抗旱、抗病等）等突变株。

• 微藻毒理研究功能：可同时测量 96 个微藻样品（对照和处理组）的光合活性，软件自动给出处理组样品相对于对照组的光合YZ百分比。

• 吸光系数测量功能：快速测量叶片的吸光系数。吸光系数测量光源： 16 个红光（650 nm）和 16 个近红外（780 nm）LED，用于测量植物叶片或藻类样品 PAR 吸光系数。

• 光强异质性：测量区域光强异质性≤±2％
  

测量参数

Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

应用领域

• 光合作用研究：可以在完全相同的条件下同时对大量样品进行成像

• 植物病理学：病斑部位（包括肉眼不可见时）成像以及病斑扩散的时空动力学

• 植物胁迫生 理学：肉眼不可见胁迫损伤的早期检测

• 遗传育种：出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株

• 突变株筛选：快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等

• 微藻毒理学：不同毒物浓度多个重复的样品一次测完，软件自动计算YZ比率

• 分子生物学：宏观水平上检测样品的绿色荧光蛋白（GFP）荧光

• 其它多种扩展研究

选购指南

一、荧光成像 MAXI 版

系统组成：通用型主机，LED 供电单元， LED 光源，CCD，数据线，软件等。

注意：高等植物或真核藻类测量时选择蓝光光源（推荐），蓝藻测量请选择红光光源。

二、荧光成像 MINI 版

系统组成：通用型主机（下图中未显示），LED 光源（如下图），CCD，数据线，软件等。

注意：高等植物或真核藻类测量时选择蓝光光源（推荐），蓝藻测量请选择红光光源。

三、荧光成像 MICROSCOPY 版

系统组成：通用型主机，特制显微镜，CCD，数据线，软件等。

产地：德国 WALZ

参考文献

数据来源：光合作用文献 Endnote 数据库，更新至 2021 年 1 月，文献数量超过 10000 篇

原始数据来源：Google Scholar

1. Salguero-Linares, J., et al. (2022). "Robust transcriptional indicators of immune cell death revealed by spatio-temporal transcriptome analyses." Molecular Plant.

2. Sandoval-Ibáñez, O., et al. (2022). "De-etiolation-induced protein 1 (DEIP1) mediates assembly of the cytochrome b6f complex in Arabidopsis." Nature communications 13(1): 4045.

3. Gao, Y., et al. (2022). "Chloroplast translational regulation uncovers nonessential photosynthesis genes as key players in plant cold acclimation." The Plant Cell.

4. Ma, L., et al. (2022). "SlRBP1 promotes translational efficiency via SleIF4A2 to maintain chloroplast function in tomato." The Plant Cell.

5. Castro, P. H., et al. (2022). "SUMO E3 Ligase SIZ1 connects sumoylation and reactive oxygen species homeostasis processes in Arabidopsis." Plant Physiology.

6. Cai, W., et al. (2022). "CaSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper." PLOS Genetics 18(2): e1010023.

7. Arsic, M., et al. (2022). "Foliar-applied manganese and phosphorus in deficient barley: Linking absorption pathways and leaf nutrient status." Physiologia plantarum n/a(n/a): e13761.

8. Ashok, A., et al. (2022). "Food-chain length determines the level of phenanthrene bioaccumulation in corals." Environmental Pollution: 118789.

9. Banc-Prandi, G., et al. (2022). "Elevated temperatures reduce the resilience of the Red Sea branching coral stylophora pistillata to copper pollution." Aquatic Toxicology 244: 106096.

10. Basit, F., et al. (2022). "Chromium toxicity induced oxidative damage in two rice cultivars and its mitigation through external supplementation of brassinosteroids and spermine." Chemosphere: 134423.

11. Brilli, F., et al. (2022). "Exposure to different light intensities affects emission of volatiles and accumulations of both pigments and phenolics in Azolla filiculoides." Physiologia plantarum n/a(n/a): e13619.

12. Brunner, C. A., et al. (2022). "Effects of climate change and light limitation on coral recruits." MARINE ECOLOGY PROGRESS SERIES 690: 65-82.

13. Che, L., et al. (2022). "Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex in Arabidopsis thaliana." 13.

14. Chen, C.-Y., et al. (2022). "Colonization of Piriformospora indica enhances insect herbivore resistance of rice plants through jasmonic acid-and antioxidant-mediated defense mechanisms." Journal of Plant Interactions 17(1): 9-18.

15. Chen, Q., et al. (2022). "Strategies of carbon use and photosynthetic performance of the two seaweeds Gracilaria chouae and Gracilariopsis lemaneiformis under different conditions of the carbonate system." Algal Research 64: 102713.

16. Crawford, A., et al. (2022). "Clay 3D printing as a bio-design research tool: development of photosynthetic living building components." Architectural Science Review: 1-11.

17. d'Aquino, L., et al. (2022). "Growth and metabolism of basil grown in a new-concept microcosm under different lighting conditions." Scientia Horticulturae 299: 111035.

18. Dahro, B., et al. (2022). "Two AT-Hook proteins regulate A/NINV7 expression to modulate sucrose catabolism for cold tolerance in Poncirus trifoliata." n/a(n/a).

19. Deguette, A., et al. (2022). "Physiological and morphological effects of a marine heatwave on the seagrass Cymodocea nodosa." Scientific Reports 12(1): 7950.

20. Emma Sumner, et al. (2022). "Acclimation to water stress improves tolerance to heat and freezing extremes in a common alpine grass." Research square.

21. Faragó, D., et al. (2022). "Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plants." Plant Cell Environ n/a(n/a).

22. Feng, Y., et al. (2022). "Growth performance, antioxidant response, biodegradation and transcriptome analysis of Chlorella pyrenoidosa after nonylphenol exposure." Science of The Total Environment 806: 150507.

23. Gao, S., et al. (2022). "The growth and photosynthetic responses of white LEDs with supplemental blue light in green onion (Allium fistulosum L.) unveiled by Illumina and single-molecule real-time (SMRT) RNA-sequencing." Environmental and Experimental Botany: 104835.

24. He, J., et al. (2022). "Salinity and Salt-Priming Impact on Growth, Photosynthetic Performance, and Nutritional Quality of Edible Mesembryanthemum crystallinum L." Plants 11(3): 332.

25. Herdean, A., et al. (2022). "Phenoplate: An innovative method for assessing interacting effects of temperature and light on non-photochemical quenching in microalgae under chemical stress." New Biotechnology 66: 89-96.

26. Hernández, K. V., et al. (2022). "Effect of light intensity on steviol glycosides production in leaves of Stevia rebaudiana plants." Phytochemistry 194: 113027.

27. Hsieh, W.-Y., et al. (2022). "THIAMIN REQUIRING2 is involved in thiamin diphosphate biosynthesis and homeostasis." The Plant Journal n/a(n/a).

28. Huang, H., et al. (2022). "BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum." Antioxidants 11(8): 1467.

29. Hunter, C., et al. (2022). "Recovery after deficiency: Systemic copper prioritization and partitioning in the leaves and stems of hybrid poplar."

30. Jin, Z., et al. (2022). "SfGPX regulates low-temperature tolerance by affecting velocity and intensity of Ca2+ transfer and photosynthetic characteristics in interfered Spiraea fritschiana and overexpressed Spiraea japonica ‘Gold Mound’." Journal of Plant Physiology 272: 153693.

31. Jung, P., et al. (2022). "Salty Twins: Salt-Tolerance of Terrestrial Cyanocohniella Strains (Cyanobacteria) and Description of C. rudolphia sp. nov. Point towards a Marine Origin of the Genus and Terrestrial Long Distance Dispersal Patterns." 10(5): 968.

32. Kaplenig, D., et al. (2022). "Repeated colonization of alpine habitats by Arabidopsis arenosa viewed through freezing resistance and ice management strategies." Plant Biology n/a(n/a).

33. Kareem, H. A., et al. (2022). "Nanosized zinc oxide (n-ZnO) particles pretreatment to alfalfa seedlings alleviate heat-induced morpho-physiological and ultrastructural damages." Environmental Pollution 303: 119069.

34. King, O. C., et al. (2022). "Individual and combined effects of diuron and light reduction on marine microalgae." Ecotoxicology and Environmental Safety 241: 113729.

35. Kőrösi, L., et al. (2022). "Phase-Selective Synthesis of Anatase and Rutile TiO2 Nanocrystals and Their Impacts on Grapevine Leaves: Accumulation of Mineral Nutrients and Triggering the Plant Defense." Nanomaterials 12(3): 483.

36. Kottuparambil, S. (2022). "Euglenoid Flagellates in Ecotoxicological Research: Progress and Perspectives. ." Preprints.

37. Li, J., et al. (2022). "Melatonin enhances the low-temperature combined low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating photosynthesis, carotenoid, and hormone metabolism." Environmental and Experimental Botany 199: 104868.

38. Li, M., et al. (2022). "The combined treatments of brassinolide and zeaxanthin better alleviate oxidative damage and improve hypocotyl length, biomass, and the quality of radish sprouts stored at low temperature." Food Chemistry: X 15: 100394.

39. Li, N., et al. (2022). "Silicon enhanced the resistance of Chinese cabbage (Brassica rapa L. ssp. pekinensis) to ofloxacin on the growth, photosynthetic characteristics and antioxidant system." Plant Physiology and Biochemistry 175: 44-57.

40. Li, T., et al. (2022). "Environmental nitrogen and phosphorus nutrient variability triggers intracellular resource reallocation in Gracilariopsis lemaneiformis (Rhodophyta)." Algal Research 66: 102778.

41. Li, W., et al. (2022). "Changes of starch and sucrose content and related gene expression during the growth and development of Lanzhou lily bulb." PLoS ONE 17(1): e0262506.

42. Li, X., et al. (2022). "The positive effects of exogenous sodium nitroprusside on the plant growth, photosystem II efficiency and Calvin cycle of tomato seedlings under salt stress." Scientia Horticulturae 299: 111016.

43. Liang, J., et al. (2022). "Dualistic effects of bisphenol A on growth, photosynthetic and oxidative stress of duckweed (Lemna minor)." Environmental Science and Pollution Research.

44. Liang, Z., et al. (2022). "Physiological and biochemical responses to light and temperature stress in free-living conchocelis of Neopyropia katadae (Bangiales, Rhodophyta)." Journal of Applied Phycology.

45. Lin, D., et al. (2022). "SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato." Plant biotechnology journal.

46. Lin, S., et al. (2022). "Exogenous melatonin improved photosynthetic efficiency of photosystem II by reversible phosphorylation of thylakoid proteins in wheat under osmotic stress." Frontiers in Plant Science 13.

47. Lintner, M., et al. (2022). "Photosynthetic performance of symbiont-bearing foraminifera Heterostegina depressa affected by sunscreens." Scientific Reports 12(1): 2750.

48. Lintner, M., et al. (2022). "Effect of light on the metabolism of the foraminifera Cribroelphidium selseyense lacking photosymbionts and kleptoplasts." Journal of Photochemistry and Photobiology 11: 100133.

49. Lips, S., et al. (2022). "Community metabolomics provides insights into mechanisms of pollution-induced community tolerance of periphyton." Science of The Total Environment: 153777.

50. Liu, B., et al. (2022). "Photoprotection contributes to freezing tolerance as revealed by RNA-seq profiling of rhododendron leaves during cold acclimation and deacclimation over time." Hortic Res 9.

51. Liu, C.-X., et al. (2022). "The E3 Ubiquitin Ligase Gene Sl1 Is Critical for Cadmium Tolerance in Solanum lycopersicum L." Antioxidants 11(3): 456.

52. Liu, K., et al. (2022). "Melatonin delays leaf senescence and improves cucumber yield by modulating chlorophyll degradation and photoinhibition of PSII and PSI." Environmental and Experimental Botany 200: 104915.

53. Liu, Y., et al. (2022). "Brassinosteroids promote starch synthesis and the implication in low-light stress tolerance in Solanum lycopersicum." Environmental and Experimental Botany 201: 104990.

54. Liu, Z., et al. (2022). "Effects of different nitrogen forms and concentrations on seedling growth traits and physiological characteristics of Populus simonii × P. nigra." Journal of Forestry Research.

55. Lopes Rezende-Silva, S., et al. (2022). "Morphophysiological indicators of the glyphosate action on Brazilian savanna plants: a multivariate analysis." Acta Physiologiae Plantarum 44(7): 73.

56. Lopez, L. S., et al. (2022). "The Arabidopsis T-DNA mutant SALK_008491 carries a 14-kb deletion on chromosome 3 that provides rare insights into the plant response to dynamic light stress." Plant Direct 6(7): e429.

57. Lu, Q., et al. (2022). "CabZIP23 Integrates in CabZIP63–CaWRKY40 Cascade and Turns CabZIP63 on Mounting Pepper Immunity against Ralstonia solanacearum via Physical Interaction." 23(5): 2656.

58. Lu, S., et al. (2022). "VvERF17 mediates chlorophyll degradation by transcriptional activation of chlorophyll catabolic genes in grape berry skin." Environmental and Experimental Botany 193: 104678.

59. Lv, C., et al. (2022). "H2O2 participates in ABA regulation of grafting-induced chilling tolerance in cucumber." Plant Cell Reports.

60. Lynch, T., et al. (2022). "ABI5 binding protein2 inhibits ABA responses during germination without ABA-INSENSITIVE5 degradation." Plant Physiology.

61. Marambio, J., et al. (2022). "Hyposalinity affects diurnal photoacclimation patterns in the rhodophyte Palmaria palmata under mimicked Arctic summer conditions." Journal of Photochemistry and Photobiology 11: 100124.

62. Maressa Hungria de Lima e Silva, I., et al. (2022). "Fluoride effect indicators in Phaseolus vulgaris seeds and seedlings." PeerJ 10.

63. Moog, M. W., et al. (2022). "The epidermal bladder cell-free mutant of the salt tolerant quinoa challenges our understanding of halophyte crop salinity tolerance." New Phytologist n/a(n/a).

64. Moustaka, J., et al. (2022). "Root-Associated Entomopathogenic Fungi Modulate Their Host Plant’s Photosystem II Photochemistry and Response to Herbivorous Insects." Molecules 27(1): 207.

65. Nazir, M. M., et al. (2022). "Exogenous calcium oxide nanoparticles alleviate cadmium toxicity by reducing Cd uptake and enhancing antioxidative capacity in barley seedlings." Journal of hazardous materials 438: 129498.

66. Nilsson, A. R., et al. (2022). "The globally threatened epiphytic cyanolichen Erioderma pedicellatum depends on a rare combination of habitat factors." The Lichenologist 54(2): 123-136.

67. Okereke, C. N., et al. (2022). "Impact of heat stress of varying severity on papaya (Carica papaya) leaves: major changes in stress volatile signatures, but surprisingly small enhancement of total emissions." Environmental and Experimental Botany: 104777.

68. Om, K., et al. (2022). "Pyruvate, phosphate dikinase regulatory protein impacts light response of C4 photosynthesis in Setaria viridis." Plant Physiology.

69. Pan, X., et al. (2022). "Transcriptional and physiological data revealed cold tolerance in a photo-thermo sensitive genic male sterile line Yu17S." BMC Plant Biology 22(1): 44.

70. Pan, Y., et al. (2022). "Physiological and metabolic analysis in mother bulbs of Lilium davidii var. unicolor during growth and development." Acta Physiologiae Plantarum 44(9): 88.

71. Pandey, K., et al. (2022). "Coordinated regulation of photosynthesis and sugar metabolism in guar increases tolerance to drought." Environmental and Experimental Botany 194: 104701.

72. Phinney, N. H., et al. (2022). "The lichen cushion: A functional perspective of color and size of a dominant growth form on glacier forelands." Fungal Biology.

73. Roach, T., et al. (2022). "Acquisition of desiccation tolerance in Haematococcus pluvialis requires photosynthesis and coincides with lipid and astaxanthin accumulation." Algal Research 64: 102699.

74. Rotasperti, L., et al. (2022). "The barley mutant happy under the sun 1 (hus1): An additional contribution to pale green crops." Environmental and Experimental Botany 196: 104795.

75. Salbitani, G., et al. (2022). "Microalgae cross-fertilization: short-term effects of Galdieria phlegrea extract on growth, photosynthesis and enzyme activity of Chlorella sorokiniana cells." Journal of Applied Phycology.

76. Salguero-Linares, J., et al. (2022). "Robust transcriptional indicators of immune cell death revealed by spatio-temporal transcriptome analyses." Molecular Plant.

77. Sarropoulou, V., et al. (2022). "The use of different LEDs wavelength and light intensities on in vitro proliferation of cherry rootstock: in fluence on photosynthetic pigments, photosystem II photochemistry and leaf anatomy."

78. Sefbom, J., et al. (2022). "Local adaptation through countergradient selection in Northern Populations of

79. Skeletonema marinoi." Evolutionary Applications.

80. Shang, Z., et al. (2022). "Enhanced potential of a Selenastrum capricornutum mutant for CO2 fixation and biofuels production." Environment Progress & Sustainable Energy. n/a(n/a): e13838.

81. Shao, G., et al. (2022). "Transcriptome Analysis Reveals Genes Respond to Chlorophyll Deficiency in Green and Yellow Leaves of Chrysanthemum morifolium Ramat." Horticulturae 8(1).

82. Shaoli, H., et al. (2022). "Cytokinin plays a critical role in bitter gourd rootstock-induced thermotolerance of cucumber." Vegetable Research 1(1): 1-9.

83. Shindo, A., et al. (2022). "Interactive effects of temperature and irradiance including spectral light quality on the photosynthesis of a brown alga Saccharina japonica (Laminariales) from Hokkaido, Japan." Algal Research 66: 102777.

84. Silva, V. N. B., et al. (2022). "Multi-omics Analysis of Young Portulaca oleracea L. Plants’ Responses to High NaCl Doses Reveals Insights into Pathways and Genes Responsive to Salinity Stress in this Halophyte Species." Phenomics.

85. Sohail, H., et al. (2022). "Genome-wide identification of plasma-membrane intrinsic proteins in pumpkin and functional characterization of CmoPIP1-4 under salinity stress." Environmental and Experimental Botany: 104995.

86. Song, W., et al. (2022). "Functional characterization and comparison of lycopene epsilon-cyclase genes in Nicotiana tabacum." BMC Plant Biology 22(1): 252.

87. Sperdouli, I., et al. (2022). "Excess Zinc Supply Reduces Cadmium Uptake and Mitigates Cadmium Toxicity Effects on Chloroplast Structure, Oxidative Stress, and Photosystem II Photochemical Efficiency in Salvia sclarea Plants." toxics 10(1): 36.

88. Sperdouli, I., et al. (2022). "Reactive Oxygen Species Initiate Defence Responses of Potato Photosystem II to Sap-Sucking Insect Feeding." Insects 13(5): 409.

89. Suriyagoda, L., et al. (2022). "Growth and nutrition of rice seedlings when phosphorus or silicon was applied to a soil heavily contaminated with both arsenic and cadmium." Journal of Plant Nutrition: 1-17.

90. Szádeczky-Kardoss, I., et al. (2022). "Elongation factor TFIIS is essential for heat stress adaptation in plants." Nucleic Acids Research.

91. Szechynska-Hebda, M., et al. (2022). "Aboveground Plant-to-Plant Electrical Signaling Mediates Network Acquired Acclimation." Plant Cell.

92. Széles, E., et al. (2022). "Microfluidic Platforms Designed for Morphological and Photosynthetic Investigations of Chlamydomonas reinhardtii on a Single-Cell Level." Cells 11(2): 285.

93. Teng, L., et al. (2022). "Effect of precipitation change on the photosynthetic performance of Phragmites australis under elevated temperature conditions." PeerJ 10: e13087.

94. Trainin, T., et al. (2022). "Physiological characterization of the wild almond Prunus arabica stem photosynthetic capability." 13.

95. Vivier, B., et al. (2022). "Colonisation of artificial structures by primary producers: competition and photosynthetic behaviour." Biofouling: 1-14.

96. Wang, C.-m., et al. (2022). "Physiological and transcription analyses reveal regulatory pathways of 6-benzylaminopurine delaying leaf senescence and maintaining quality in postharvest Chinese flowering cabbage." Food Research International 157: 111455.

97. Wang, H., et al. (2022). "Regulatory interaction of BcWRKY33A and BcHSFA4A promotes salt tolerance in non-heading Chinese cabbage [Brassica campestris (syn. Brassica rapa) ssp. chinensis]." Horticulture research.

98. Wang, Q., et al. (2022). "The NADPH oxidase OsRbohA increases salt tolerance by modulating K+ homeostasis in rice." The Crop Journal.

99. Wardley, W. P., et al. (2022). "Measuring Photonics in Photosynthesis: Combined Micro-Fourier Image Spectroscopy and Pulse Amplitude Modulated Chlorophyll Fluorimetry at the Micrometre-Scale." biomimetics 7(3): 107.

100. Wlodkowic, D. and M. Jansen (2022). "High-throughput screening paradigms in ecotoxicity testing: Emerging prospects and ongoing challenges." Chemosphere 307: 135929.