运用高速逆流色谱从浙贝母分离纯化贝母碱和去氢贝母碱
2007-01-15846
文件大小:184.27KB
建议WIFI下载,土豪忽略
方案优势
Abstract: High-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied to preparative separation and purification of verticine and verticinone from crude extracts of Bulbus Fritillariae Thunbergii by a one-step separation,using chloroformethanol0.2 mol L−1 hydrochloric acid (3:2:2, v/v/v) as a solvent system. HPLC analysis of the fractions collected on the preparative HSCCC of 200 mg of crude extracts showed that the purity of verticine (25.6 mg) was 96.8% and that of verticinone (10.3 mg) was 95.4%. The chemical identities of these components were confirmed by H-NMR and EI-MS. TBE-1000A制备色谱仪/萃取仪/快速分离制备色谱仪 TBE300B+AKTA高速逆流色谱仪/离心分配色谱/萃取仪/制备色谱仪 TBE-200V 高速逆流色谱仪
相关产品
选购仪器 上yiqi.com
仪器网络推广
品牌网上传播
长按识别二维码查看信息详情