Application of preparative high-speed counter-curren
2007-01-11478
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Abstract: Following an initial cleaning-up step on the D101 macroporous resin,a preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexaneethyl acetatemethanolwater (1:0.9:0.9:1, v/v) was used to isolate and separate schizandrin and gomisin A from Schisandra chinensis. A total of 107 mg schizandrin and 36 mg gomisin A with purities of 99.5% and 99.1% were obtained from 400 mg crude extract in one-step elution and less than 3h,and the structure identification was performed by UV, IR, MS,H-NMR and C-NMR. TBE-300A高速逆流色谱仪/制备色谱仪 TBE-1000A制备色谱仪/萃取仪/快速分离制备色谱仪 TBE300B+AKTA高速逆流色谱仪/离心分配色谱/萃取仪/制备色谱仪 TBE-200V 高速逆流色谱仪
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