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The phycobilisomes were isolated from blue2green alga Spirulina platensis, and could form monolayer film at airPwater interface. The monolayer film of phycobilisomes was transferred to newly cleaved mica, and coated with gold. Scanning tunne领 microscope was used to investigate the structure of the Langmuir2Blodgett film of phycobilisomes. It was shown that phycobilisomes in the monolayer arrayed in rows with core attaching on the substrate surface and rods radiating towards the air phase, this phenomenon was similar to the arrangement of phycobilisomes on cytoplasmic surface of thylakoid membrane in vivo. The possible applications of the Langmuir2Blodgett film of phycobilisomes were also discussed.
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http://www.spm.com.cn/papers/p20.pdf 扫描探针显微镜(SPM/AFM/STM)
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