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Scanning tunne领 microscope was used to investigate the in vit ro assembly of R2 phycoerythrin (R2PE) f rom the marine red alga Polysi phonia urceolata. The result s showed that R2 PE molecules assembled together by disc2to2disc while absorbing on HOPG surface, which just looked like the rods in the phycobilisomes. When the water2soluble R2PE was dissolved in 2 % ethanol/ water spreading solution, they could form monolayer film at the air/ water interface. Similar disc2to2disc array of R2PE was constituted in the two2dimensional Langmuir2Blodgett film by the external force. It could be concluded that, apart from the key role of the linker polypeptides, the in vivo assembly of phycobiliproteins into phycobilisomes is also dependent on the endogenous properties of phycobiliprotein themselves.
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http://www.spm.com.cn/papers/p24.pdf 扫描探针显微镜(SPM/AFM/STM)