光片显微镜的显著特征是照明和成像两个物镜垂直放置。其中物镜了工作距离,成像效果等。为了更好的成像效果昊量光电推出了专用于光片成像的物镜
昊量光电目前提供两种物镜
1) 多浸渍物镜
该浸入式/浸渍式物镜旨在用于组织成像的光片显微镜系统上,并且由于该物镜较长的工作距离和RI的匹配性也通常用于活细胞成像。该物镜的主要特点是由于其独特的第1弯曲表面,可以无需校正环用于多种成像介质中。该物镜目前提供两种型号第1代物镜54-10-12 和第二代物镜54-10-8两款相比54-10-8拥有较高的分辨率和NA但是损失了工作距离和视野面积。
2) 适用于高分辨率单物镜光片显微镜物镜(AMS-AGY)
该物镜主要用于单物镜成像光片系统,
产品参数:
Specifications | Value 54-10-12 | Value 54-12-8 | Comments |
Numerical Aperture | 0.4 @ RI 1.45 | 0.7 @ RI 1.45 | NA proportional to RI |
Immersion Media RI | 1.33 – 1.56 | 1.33- 1.56 | Includes all major clearing solutions |
Effective Focal Length | 12 mm @ RI 1.45 | 8.4 mm @ RI 1.45 | Magnification proportional to RI |
Working Distance | 12 mm (for all RI) | 10.0 mm (for all RI) | |
Flat Sample Depth | 5.1 mm | 2.0 mm | Imaging depth into flat sample at 45 degree tilt |
Field of View | 1.2 mm Ø | 1.0 mm Ø | |
Spherical Correction | 480 – 1000 nm | 480 – 1300 nm | Diffraction-limited for most media and λ |
Chromatic Correction | 480 – 720 nm | 480 – 720 nm | Performance varies by media, optimized for CLARITY and TDE |
Correction Collar | None | None | Designed for immersion w/o coverslip |
Parfocal Length | 61.6 mm | 83 mm | Both have M25 threads |
AMS-AGY物镜参数:
Specifications | Value |
Numerical Aperture | 1.0 |
Effective Focal Length | 5 mm |
Working Distance | 0.0 mm |
Chromatic Correction | 450 – 700 nm |
Field (diffraction-limited) | 150 μm Ø |
Field (bevel-limited) | 250 μm Ø |
上海昊量光电设备有限公司
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