qPCR预混液

iTaq Universal Supermixes 包含 SYBR? Green 或探针法即用型预混液， 采用优化的配方适合常规 qPCR 实验。 ■ 采用抗体法快速热启动 iTaq 聚合酶快速激活反应 ■ 兼容快速和普通反应程序 ■经实验验证通用于各品牌仪器，无需单独加 ROX 染料 ■ 不同批次获得结果的重复性高 ■配合 Bio-Rad PrimePCR? Assays 效果更佳 – 经过实验验证 适合的应用： ■质粒、纯化的 gDNA 和 cDNA 样品 ■ 探针法可进行双重扩增 ■基因表达、分型、突变检测 ■信号通路分析 ■ SNP 分型 ■ 病原体检测 iTaq? universal SYBR? Green supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, SYBR? Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and fluorescein). iTaq? universal SYBR? Green supermix lets you: Obtain reliable and reproducible gene expression analysis results Amplify genomic DNA and difficult amplicons with superior efficiency Achieve consistent results across real-time instrument platforms under fast or standard conditions iTaq? universal SYBR? Green supermix contains: Advanced buffer formulation with SYBR? Green I for optimal qPCR performance Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec) Blend of passive reference dyes for universal real-time instrument compatibility Applications and Uses Gene expression analysis Genotyping Mutation detection Pathogen detection