2011年,《自然方法》杂志( Nature Methods )将单细胞测序列为年度值得期待的技术之一,2013年,《科学》杂志(Science)将单细胞测序列为年度最值得关注的六大领域榜首,表明单细胞测序已成为科研热点。应广大客户的强烈需求,英国Dolomite公司成功研发出RNA单细胞测序标准系统,并成功完成了实验,发行了应用报告。
(Macosko E., et al. "Highly Parallel Genome-wide Expression
Profi领 of Individual Cells Using Nanoliter Droplets." Cell 161:1202
Tens of thousands of single cells are encapsulated with uniquely barcoded mRNA-capture beads. The cells are lysed in the droplets, and beads capture mRNA from single cells. The single cell RNA-beads are recovered and processed for downstream high throughput sequencing.
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