Fluorescence Bottom Reading for Cell-Based Assays
2010-04-28553
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Cell-based applications are central to life science research.
They may range from cytotoxicity, proliferation, apoptosis and
GPCR signa领 assays to high-throughput screening (HTS)
drug discovery applications.
Adherent cell types are typically analyzed through the well
bottom in order to bring the cell monolayer as close as
possible to the detector and avoid unspecific fluorescence
noise by the overlying growth medium. For this reason it is
essential to guarantee illumination and reading of the entire
well bottom, since cells are not always homogeneously
distributed over the growth surface.
the present application note is to assess the
performance of the improved, fluorescence bottom fiber of the
new Infinite 200 PRO in the detection of GFP-transfected
cells. Measurement characteristics like well-to-well uniformity
and reading reproducibility were investigated. Infinite® 200 Pro多功能微孔板检测仪
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