全自动外泌体荧光检测分析系统

全自动外泌体荧光检测分析系统

应用方向及主要特征

ExoView™ 参数信息:

- 颗粒大小分辨范围：大于50 nm（可分析大于40 nm的病毒颗粒）

- 荧光粒径分辨范围：大于20 nm

- 所需样本体积：25 μL

- 激发波长：410 nm，488 nm，555 nm，640 nm

- 可一次检测16个样本，每个样本可同时检测6个不同亚型及3种生物标记的荧光定位

- 单个样品检测时间：8分钟

- 捕获抗体：一个芯片多允许6种捕获抗体（+阴性对照）

- 荧光通道：3个荧光通道

ExoView™ Kit 配置清单:

粒径分析

       Exoview能够对>50 nm的外泌体进行全方面的表征，无论是粒径尺寸、粒径分布还是外泌体的亚型均可在一次测试中得到。并且所用来测试的样本无需进行纯化，避免因纯化带来的样本偏差。

分析不同大小和不同尺度的外泌体亚群，在CD171过表达系统中，100 nm以上的外泌体仅表达了CD171。

量化外泌体亚群 

       通过抗体捕获的模式，Exoview能够量化含有不同标记物的外泌体亚群，并对不同种群的外泌体进行特异性计数和分析。整个过程无需纯化，并且线性范围可跨越3个数量级。

TSPAN8阳性细胞外囊泡的稀释曲线。使用1:3比例的梯度稀释。用R2 = 0.9986计算与TSPAN8荧光数据的线性拟合。

检测外囊泡中的装载物 

       MISEV建议在表征外泌体和外囊泡时，应当同时测量表面和载体蛋白。使用ExoView芯片可穿透外泌体，探测膜内蛋白和载体。并且单次可定量3种表面、管腔蛋白。

检测细胞外泌体内的Syntenin。

WT细胞在未穿膜的条件下几乎观测不到Syntenin的信号。进行穿膜处理后可以观测到Syntenin信号，而KO之后信号消失

生物标志物共定位 

       ExoView能够在单个外泌体样本上检测多达4种标记物，并同时提供外泌体的其它表征数据诸如计数、颗粒尺寸统计等。

无需纯化

       ExoView只测量含有靶标抗原的特定细胞外泌体群体。仅需35ul的稀释样品，即可直接获得外泌体的表型、l粒径和计数信息。并且无需担心污染物对测试的影响。

无论纯化与否，Exoview均可进行分析

相关视频

发表文章

2020年已发表文献

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2. Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy, Cho K, Kook H, Kang S Lee J, JEV 2020

3. Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis, Rogers MA, Buffolo F, Schlotter F, Atkins SK, Lee LH, Halu A, Blaser MC, Tsolaki E Higashi H, Luther K, Daaboul G, Bouten CVC, Body SC, Singh SA, Bertazzo S, Libby P, Aikawa M, Aikawa E, Cell Biology 2020

4. Targeting tumor-derived exosomes using a lectin affinity hemofiltration device, Marleau AM, Jacobs MT, Gruber N, Rodell TC, Ferrone S, Whiteside TL, Cancer Research 2020

5. Extracellular Vesicle and Particle Biomarkers DefineMultiple Human Cancers. Hoshino A, Kim HS, Bojmar L, Gyan KE, Cioffi M, Hernandez J, Zambirinis CP, Rodriques G, Molina H, Heissel S, Mark MT, Steiner L, Benito Martin A, Lucotti S, Di Giannatale A, Offer K, Nakajima M, Williams C, Lyden D., Cell 2020

6. Reporter mice for isolating and auditing cell type‐specific extracellular vesicles in vivo, McCann JV, Bischoff SR, Zhang Y, Cowley DO, Sanchez-Gonzalez V, Daaboul GG, Dudley AC, Genesis 2020

7. High yield and scalable EV production from suspension cells triggered by turbulence in a bioreactor, Grainger A, Wilhelm C, Gazeuah F, Silva A, Cytotherapy 2020

8. Extracellular Vesicles: A New Frontier for Research in Acute Respiratory Distress Syndrome, Mahida RY, Matsumoto S, Matthay MA. American Journal of Respiratory Cell and Molecular Biology 2020

9. Small extracellular vesicles modulated by αVβ3 integrin induce neuroendocrine differentiation in recipient cancer cells, Quaglia F, Krishn SR, Daaboul GG, Sarker S, Pippa R, Domingo-Domenech J, Kumar G, Fortina P, McCue P, Kelly WK, Beltran H, Liu Q, Languino LR., Journal of Extracellular Vesicles, Volume 9, Issue 1, 2020

10. Characterisation of extracellular vesicles surface markers and co-expression studies with single particle interferometric imaging platform, Kusuma G, Lim R., Cytotherapy, Volume 22, Issue 5, 2020

11. Engineering mesenchymal stem cell paracrine activity with 3D culture, Kusuma G, LiA, Zhu D, McDonald H, Chmabers D, Frith J, Lim R.,Cytotherapy, Volume 22, Issue 5, 2020

12. Release of extracellular vesicle miR-494-3p by ARPE-19 cells with impaired mitochondria,Ahn JY, Datta S, Cano M, Mallick E, Rai U, Powell B, Tian J, Witwer KW, Handa JT, Paulaitis ME.,Biochim Biophys Acta Gen Subj. 2020 Mar 30:129598. (doi: 10.1016/j.bbagen.2020.129598)

13. Advantageous Antibody Microarray Fabrication Through DNA-Directed Immobilization: A Step Toward Use of Extracellular Vesicles in Diagnostics, Brambila D, Sola L, Chiari M., Talanta 2020

14. Unannotated small RNA clusters in circulating extracellular vesicles detect early stage liver cancer, Villanueva et al, bioRxiv preprint 2020 (doi: https://doi.org/10.1101/2020.04.29.066183)

15. Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis, Gori A, Romanato A, Bergamaschi G, Strada A, Gagni P, Frigerio R, Brambilla D, Vago R, Galbiati S, PIcciolini S, Bedoni M, Daaboul G, Chiari M, Cretich M., Journal of Extracellular Vesicles, Volume 9, Issue 1, 2020

16. Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation, Crescitelli R, L?sser C, Jang SC, Cvjetkovic A, Malmh?ll C, Karimi N, H??g JL, Johansson I, Fuchs J, Thorsell A, Gho YS, Bagge RO, L?tvall J, Journal of Extracellular Vesicles , 2020

17. Extracellular Vesicles Derived from Induced Pluripotent Stem Cells Promote Renoprotection in Acute Kidney Injury Model, Collino F, Lopes JA, Tapparo M, Tortelote GG, Kasai-Brunswick TH, Lopes GMC, Almeida DB, Skovronova R, Wendt CHC, de Miranda K, Bussolati B, Vieyra A, Soares Lindoso R., Cells, 2020

18. Extracellular Vesicles: A New Frontier for Research in Acute Respiratory Distress Syndrome，Mahida RY, Matsumoto S, Matthay MA. American Journal of Respiratory Cell and Molecular Biology, 2020

19. Exosomes A clinical COmpendium - Methods for exosome isolation and characterization，Zhou M, Weber SR, Zhao Y, Chan H, Sundstrom JM. Exosomes A Clinical Compendium 2020

20. Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence，Panagopoulou MS, Wark AW, Birch DJS, Gregory CD. Journal of Extracellular Vesicles, 2020

21. Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle Yan W, Jiang S, Trends in Cancer 2020