资料库
仪器网 >
资料库 >The Solartron CellTest® System
The Solartron CellTest® System
本文由 阿美特克商贸（上海）有限公司 整理汇编
2024-09-28 06:05 826阅读次数
文档仅可预览首页内容，请下载后查看全文信息！
立即下载
The Solartron CellTest® System
更多资料
The Solartron CellTest® System The Solartron CellTest® System[详细]
2024-09-28 06:05
实验操作
Solartron电化学产品样本 Solartron电化学产品样本[详细]
2024-09-27 06:34
操作手册
Solartron电源领域产品样本 Solartron电源领域产品样本[详细]
2007-03-29 00:00
实验操作
Solartron材料领域产品样本 Solartron材料领域产品样本[详细]
2024-09-28 01:07
应用文章
Solartron频率响应分析仪产品样本 Solartron频率响应分析仪产品样本[详细]
2024-09-29 05:49
课件
Burst Dispensing System With an already flourishing grinder-polisher family, Buehler has expanded and simplified capabilities by introducing the BurstDispensing System.[详细]
2021-02-22 21:34
产品样册
Apex. S System The Apex S Quick Change System improves productivity and usability of Grinding-Polishing machines through simple application and effortless removal of grinding surfaces to the working platen.[详细]
2021-02-23 10:51
产品样册
AutoMet™ Grinder-Polisher System The AutoMet grinder polishers are high performance machines designed for reliability, flexibility, and ease of use.[详细]
2021-02-23 10:56
产品样册
Respiration Activity MOnitoring System Respiration Activity MOnitoring System[详细]
2008-05-05 00:00
产品样册
AxION DSA System（PerkinElmer） AxION DSA System（PerkinElmer）[详细]
2014-08-12 00:00
产品样册
brochure of gel documentation system brochure of gel documentation system[详细]
2008-08-15 00:00
选购指南
日本NK System培养箱 日本NK System培养箱[详细]
2009-01-14 00:00
报价单
30mm Cage System U-Bench 30mm Cage System U-Bench[详细]
2011-02-17 00:00
标准
Mixture/Dose Delivery System Mixture/Dose Delivery System[详细]
2014-09-24 00:00
专利
5 Stage Preparation System 5 Stage Preparation System[详细]
2012-05-08 00:00
专利
BD RevTet System使用说明 BDRevTetSystem使用说明pRevTet-On，pRevTet-Off，pRevTet-Off-IN，pRevTREIntroductionTheBDRevTet-OnTM&BDRevTet-OffTMSystemscombinetheadvantagesofretrovirus-mediatedgenetransferwiththeproventetracycline-regulatedcontrolofBDTet-OnTMandBDTet-OffTMGeneExpressionSystems.BDRevTetTMallowsthefastandefficientestablishmentofregulatedgeneexpressionsystemsinawidevarietyofcelltypes.EachRevTetSystemisacompleteretroviralgeneexpressionsystemcontainingaretroviralregulator（r）tTAvector,aretroviralresponsevector,acontrolvector,andapackagingcellline.LikeourBDRetro-XTMSystem（#631508）,theRevTetSystemscontaintheBDRetroPackTMPT67PackagingCellLine（#631510）forthesafe,efficientproductionofinfectious,replication-incompetentretrovirus.TheRevTetSystemsprovideapowerfulandconvenientapproachtosettingupthetetracycline（Tc）-inducible,high-levelgeneexpressionsystemsfirstdescribedbyGossenandBujard（1992）.Retroviral-mediatedtet-regulationTheTetSystemsarebasedontwoelementsfromthetetoperonoftheE.coliTn10transposontheTetrepressorprotein（TetR）andthetetoperatorDNAsequence（tetO）.ThegenetobeexpressedisclonedintothepRevTREresponsevectordownstreamofthetetracycline-responsiveelement（TRE）,whichcontainssevendirectrepeatsofthe42-bptetOsequenceandtheminimalimmediateearlypromoterofcytomegalovirus（PminCMV）.Thetwosystemsdifferintheirregulatoryvector（Figure1）.TheRevTet-OffSystemusesthepRevTet-OffVector,whichcontainsthetTAregulatoryelement,afusionofTetRandtheVP16activationdomain.TheBDRevTet-OnTMSystemusespRevTet-On,basedonthereversetTA（rtTA）.BothsystemsusethepRevTREresponsevector.AlltheRevTetVectorsarederivedfrompLNCX,aBDRetro-XTMvectorcapableofproducinghigh-titervirusintheappropriatepackagingcelllines.IntheRevTet-OffSystem,tTAbindstheTREandactivatestranscriptionintheabsenceofTcortheTcderivativedoxycycline（Dox）.Thus,asTcorDoxisaddedtotheculturemedium,transcriptionisturnedoffinadose-dependentmanner（Figure1）.Conversely,theRevTet-OnSystemallowsgeneexpressiontobeactivatedbytheadditionofDox.Thesetwocomplementarysystemsallowyoutochoosetheonethatbestsuitsyourneeds.BenefitsandapplicationsofTc-mediatedinductionInmostinduciblemammaliangeneexpressionsystems（e.g.,inductionbyheavymetals,steroidhormones,orheatshock）,inductionisnonspecificandexpressionlevelscannotbepreciselyregulated.Inaddition,thesesystemsaregenerallyleakyinthe“off”state,andtheinducingagentitselfmaybecytotoxicorhavepleiotropiceffectsonresults.Incontrast,regulationofgeneexpressionbytheheterologousbacterialcontrolelementsintheRevTetSystemsisveryspecific.Furthermore,thelevelsofTcorDoxrequiredforthefullrangeofgeneexpressionarenotcytotoxicandhavenosignificanteffectoncellproliferationoranimalgrowth,evenwithcontinuoustreatment（Bohletal.,1997;Mayfordetal.,1996）.TheuseofseparateregulatoryandresponsevectorsintheRevTetSystems质粒图谱:[详细]
2018-09-29 10:03
产品样册
Turnkey system产品手册 Turnkey system产品手册[详细]
2020-09-11 16:27
标准
POEM system产品手册 POEM system产品手册[详细]
2020-09-11 16:29
标准
SimpliVac Advanced Vacuum System The SimpliVac provides superior pore impregnation in an easy to use and compact format.[详细]
2021-02-23 15:30
产品样册
PetroThin™ Thin Sectioning System The PetroThin Thin Sectioning System is a precise, easy-to-use instrument for re-sectioning and thinning a wide variety of samples,such as rocks and minerals, ceramics, concrete, bone, and teeth for performing materials characterization.[详细]
2021-02-24 14:57
产品样册
Copyright 2004-2024 yiqi.com All Rights Reserved , 未经书面授权 , 页面内容不得以任何形式进行复制
The Solartron CellTest® System
brochure of gel documentation system
在线留言
沪公网安备 31011502008050号
参与评论
登录后参与评论