描述 Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 100K, and can be used for processing sample volumes up to 0.5 mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
• Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
• Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
• Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.
Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.
Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.
Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL
For Research Use Only. Not for use in diagnostic procedures.
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Separation of polymers from small molecules using Protein Concentrators PES, 100K MWCO
The excipient, sodium starch glycolate (MW > 500,000 to 11,000,000), was separated from a mixture of protease inhibitors leupeptin (MW 475.6), AEBSF (MW 239.69), and bestatin (MW 345) using the Pierce Concentrator PES, 100K MWCO, 0.5mL. The excipient and the inhibitors with exicpient were dissolved in 0.15M NaCl. Half of the volume was loaded into the concentrator after pre-wetting the membrane with 0.15M NaCl. Samples were centrifuged for 10 minutes at 13,000 x g. The flow-through was collected and compared to the non-concentrated controls using reverse-phase HPLC. HPLC conditions: column = Synergi 4u Hydro-RP 80A, Gradient Buffer A = 0.1% TFA in water; Gradient Buffer B = 0.1% TFA in acetonitrile. Panel A. HPLC chromatogram of the excipient and protease inhibitor mixture. Panel B. HPLC chromatogram of the mixture after removal of the excipient using the concentrator. Peaks are identified and labeled." data-omni-action="View figure"> Separation of polymers from small molecules using Protein Concentrators PES, 100K MWCO
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