NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心，供应数十万种质粒、载体、菌种、基因、细胞、感受态细胞等产品。NTCC典型培养物保藏中心-BioVector质粒载体菌种细胞基因保藏中心资源种类齐全，涵盖标准菌种、质控菌株、食品检测菌种、药品检测菌种、环境监测菌种、NTCC/DSMZ/ATCC菌种细胞、克隆载体、真核表达载体、原核表达载体、动物细胞表达载体、慢病毒载体、腺病毒逆病毒载体、RNAi基因沉默基因敲除载体、抗体表达载体GS加压筛选悬浮细胞表达系统、信号通路报告载体、亚细胞定位载体、荧光蛋白载体、昆虫细胞/杆状病毒表达载体、植物表达载体RNAi干扰沉默载体、农杆菌菌株与感受态细胞、启动子载体、诱导调控载体、毕赤酵母/酿酒酵母表达载体、酵母表面展示系统、酵母单杂双杂三杂交系统、噬菌体展示抗体系统、枯草芽孢杆菌表达载体系统、乳酸菌表达系统、分支杆菌表达系统、假单胞菌表达载体基因敲除质粒、细胞自噬质粒载体LC3、细胞永生化质粒载体SV40/hTERT、iPS干细胞重编程载体系统、转座子载体、CRISPR/Cas9载体系统TALEN基因编辑系统、慢病毒荧光表达载体、果蝇/线虫表达载体、革兰氏阳性菌/金葡菌/链霉菌链球菌表达载体敲除载体、稀有基因合成克隆等几十种类型数十万个品种。并可提供质粒/菌种保藏鉴定、基因合成、基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等实验技术服务。BioVector NTCC Inc.还可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。
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NCI-H1963 [H1963] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5982 NCI-H1963 [H1963] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5982
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H1975 [H-1975, H1975] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5908 NCI-H1975 [H-1975, H1975] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5908
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H1993 [H1993] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5909 NCI-H1993 [H1993] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5909
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2009 [H2009] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5911 NCI-H2009 [H2009] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5911
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2023 [H2023] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5912 NCI-H2023 [H2023] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5912
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2029 [H2029] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5913 NCI-H2029 [H2029] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5913
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2030 [H2030] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5914 NCI-H2030 [H2030] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5914
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2052 [H2052] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5915 NCI-H2052 [H2052] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5915
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2066 [H2066] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5917 NCI-H2066 [H2066] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5917
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2073 [H2073] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5918 NCI-H2073 [H2073] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5918
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2081 [H2081] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5920 NCI-H2081 [H2081] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5920
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2085 [H2085] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5921 NCI-H2085 [H2085] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5921
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2087 [H2087] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5922 NCI-H2087 [H2087] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5922
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H209 [H209] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-172 NCI-H209 [H209] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-172
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2106 [H2106] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5923 NCI-H2106 [H2106] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5923
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2107 [H2107] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5983_FL NCI-H2107 [H2107] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5983_FL
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2108 [H2108] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5984 NCI-H2108 [H2108] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5984
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H211 [H211] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5824 NCI-H211 [H211] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5824
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2110 [H2110] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5924 NCI-H2110 [H2110] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5924
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2122 [H2122] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5985 NCI-H2122 [H2122] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5985
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2126 [H2126] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-256 NCI-H2126 [H2126] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-256
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2135 [H2135] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5926 NCI-H2135 [H2135] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5926
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2141 [H2141] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5927 NCI-H2141 [H2141] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5927
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2170 [H2170] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5928 NCI-H2170 [H2170] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5928
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2171 [H2171] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5929 NCI-H2171 [H2171] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5929
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2172 [H2172] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5930 NCI-H2172 [H2172] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5930
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2195 [H2195] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5931 NCI-H2195 [H2195] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5931
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2196 [H2196] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5932 NCI-H2196 [H2196] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5932
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2198 [H2198] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5933 NCI-H2198 [H2198] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5933
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H220 [H220] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5825 NCI-H220 [H220] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5825
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2227 [H2227] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5934 NCI-H2227 [H2227] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5934
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2228 [H2228] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5935 NCI-H2228 [H2228] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5935
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H226 [H226] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5826 NCI-H226 [H226] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5826
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2286 [H2286] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5938 NCI-H2286 [H2286] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5938
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2291 [H2291] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5939 NCI-H2291 [H2291] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5939
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H23 [H23] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5800 NCI-H23 [H23] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5800
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2330 [H2330] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5940 NCI-H2330 [H2330] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5940
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2342 [H2342] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5941 NCI-H2342 [H2342] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5941
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2347 [H2347] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5942 NCI-H2347 [H2347] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5942
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2405 [H2405] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5944 NCI-H2405 [H2405] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5944
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2444 [H2444] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5945 NCI-H2444 [H2444] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5945
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H2452 [H2452] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5946 NCI-H2452 [H2452] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5946
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H250 [H250] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5828 NCI-H250 [H250] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5828
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H28 [H28] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5820 NCI-H28 [H28] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5820
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H292 [H292] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1848 NCI-H292 [H292] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1848
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H295R [H295R] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2128 NCI-H295R [H295R] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2128
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H345 [H345] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-180 NCI-H345 [H345] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-180
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H358 [H-358, H358] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5807 NCI-H358 [H-358, H358] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5807
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H378 [H378] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5808 NCI-H378 [H378] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5808
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H441 [H441] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-174 NCI-H441 [H441] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-174
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H446 [H446] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-171 NCI-H446 [H446] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-171
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H460 [H460] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-177 NCI-H460 [H460] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-177
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H498 [H498] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-254 NCI-H498 [H498] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-254
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H508 [H508] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-253 NCI-H508 [H508] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-253
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H510A [H510A, NCI-H510] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-184 NCI-H510A [H510A, NCI-H510] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-184
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H520 [H520] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-182 NCI-H520 [H520] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-182
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H522 [H522] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5810 NCI-H522 [H522] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5810
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H524 [H524] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5831 NCI-H524 [H524] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5831
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H526 [H526] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5811 NCI-H526 [H526] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5811
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H596 [H596] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-178 NCI-H596 [H596] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-178
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H64 [H64] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5976 NCI-H64 [H64] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5976
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H647 [H647] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5834 NCI-H647 [H647] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5834
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H650 [H650] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5835 NCI-H650 [H650] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5835
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H660 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5813 NCI-H660 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5813
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H661 [H661] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-183 NCI-H661 [H661] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-183
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H676B [H676B] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-179 NCI-H676B [H676B] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-179
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H69 [H69] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-119 NCI-H69 [H69] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-119
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H711 [H711] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5836_FL NCI-H711 [H711] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5836_FL
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H716 [H716] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-251 NCI-H716 [H716] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-251
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H719 [H719] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5837 NCI-H719 [H719] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5837
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H720 [H720] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5838 NCI-H720 [H720] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5838
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H727 [H727] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5815 NCI-H727 [H727] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5815
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H735 [H735] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5978 NCI-H735 [H735] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5978
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H740 [H740] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5840 NCI-H740 [H740] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5840
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H747 [H747] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-252 NCI-H747 [H747] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-252
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H748 [H748] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5841 NCI-H748 [H748] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5841
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H774 [H774] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5842 NCI-H774 [H774] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5842
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H810 [H810] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5816 NCI-H810 [H810] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5816
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H82 [H82] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-175 NCI-H82 [H82] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-175
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H820 [H820] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-181 NCI-H820 [H820] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-181
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H835 [H835] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5843 NCI-H835 [H835] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5843
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H838 [H838] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5844 NCI-H838 [H838] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5844
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H841 [H841] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5845 NCI-H841 [H841] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5845
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H847 [H847] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5846 NCI-H847 [H847] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5846
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H865 [H865] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5849 NCI-H865 [H865] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5849
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H889 [H889] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5817 NCI-H889 [H889] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5817
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H920 [H920] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5850 NCI-H920 [H920] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5850
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-H929 [H929] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-9068 NCI-H929 [H929] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-9068
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NCI-N87 [N87] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5822 NCI-N87 [N87] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5822
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Neo Jurkat cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2898 Neo Jurkat cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2898
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NIH:OVCAR-3 [OVCAR3] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-161 NIH:OVCAR-3 [OVCAR3] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-161
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NK-92 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2407 NK-92 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2407
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NK-92MI cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2408 NK-92MI cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2408
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NL20 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2503 NL20 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2503
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NL20-TA [NL20T-A] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2504 NL20-TA [NL20T-A] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2504
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NM2C5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2918 NM2C5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2918
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NM2C5 GFP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2919 NM2C5 GFP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2919
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
No Per cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1327 No Per cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1327
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NRAS mutant-A375 Isogenic Cell Line cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心CRL-1619IG-2 NRAS mutant-A375 Isogenic Cell Line cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心CRL-1619IG-2
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NTERA-2 cl.D1 [NT2/D1] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1973 NTERA-2 cl.D1 [NT2/D1] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1973
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
NU-DUL-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2969 NU-DUL-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2969
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
OAT1 HEK 293T/17 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11268G-1 OAT1 HEK 293T/17 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11268G-1
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Or De cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1366 Or De cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1366
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Os Te cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1262 Os Te cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1262
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
OV-90 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11732 OV-90 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11732
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
P116 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2676 P116 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2676
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
P116.cl39 [P116.c39] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2677 P116.cl39 [P116.c39] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2677
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
P3HR-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-62 P3HR-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-62
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Pa Kel-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1341 Pa Kel-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1341
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Pa Kel-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1344 Pa Kel-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1344
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PA-1 [PA1] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1572 PA-1 [PA1] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1572
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Panc 02.03 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2553 Panc 02.03 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2553
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Panc 02.13 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2554 Panc 02.13 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2554
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Panc 03.27 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2549 Panc 03.27 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2549
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Panc 04.03 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2555 Panc 04.03 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2555
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Panc 05.04 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2557 Panc 05.04 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2557
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Panc 08.13 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2551 Panc 08.13 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2551
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Panc 10.05 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2547 Panc 10.05 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2547
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PANC-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1469 PANC-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1469
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PC-3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1435 PC-3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1435
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PEAKrapid cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2828 PEAKrapid cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2828
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Per Sel cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1107 Per Sel cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1107
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Pfeiffer cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2632 Pfeiffer cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2632
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PFSK-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2060 PFSK-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2060
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Pharynx Pharynx
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PHM1-41 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3046 PHM1-41 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3046
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Phoenix-AMPHO cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3213 Phoenix-AMPHO cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3213
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Phoenix-ECO cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3214 Phoenix-ECO cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3214
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Phoenix-GP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3215 Phoenix-GP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3215
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PL45 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2558 PL45 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2558
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PLC/PRF/5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8024 PLC/PRF/5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8024
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PMC2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-9852 PMC2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-9852
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
ProPak-A.52 Clone #52 [PP-A.52] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-12479 ProPak-A.52 Clone #52 [PP-A.52] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-12479
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
ProPakA.6 [PPA.6, ProPak-A.6] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-12006 ProPakA.6 [PPA.6, ProPak-A.6] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-12006
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
ProPak-X.36 [PP-X.36] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-12007 ProPak-X.36 [PP-X.36] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-12007
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PSN-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3211 PSN-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3211
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PWR-1E cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11611 PWR-1E cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11611
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
PZ-HPV-7 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2221 PZ-HPV-7 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2221
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ra Bot cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1265 Ra Bot cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1265
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ra Lot cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1289 Ra Lot cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1289
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Raji cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-86 Raji cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-86
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ramos (RA 1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1596 Ramos (RA 1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1596
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ramos.2G6.4C10 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1923 Ramos.2G6.4C10 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1923
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ran De cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1287 Ran De cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1287
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ray Hot cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1226 Ray Hot cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1226
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RD cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-136 RD cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-136
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RD-ES cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-166 RD-ES cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-166
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Reh cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8286 Reh cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8286
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RKO cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2577 RKO cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2577
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RKO-AS45-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2579 RKO-AS45-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2579
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RKO-E6 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2578 RKO-E6 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2578
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RL cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2261 RL cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2261
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RL95-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1671 RL95-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1671
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ro Bon cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1248 Ro Bon cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1248
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ro Dow cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1337 Ro Dow cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1337
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ro Shi cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1310 Ro Shi cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1310
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ro Vid cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1308 Ro Vid cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1308
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ron Har cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1131 Ron Har cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1131
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RPMI 1788 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-156 RPMI 1788 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-156
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RPMI 2650 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-30 RPMI 2650 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-30
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RPMI 6666 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-113 RPMI 6666 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-113
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RPMI 7666 [Gerner 7666] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-114 RPMI 7666 [Gerner 7666] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-114
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RPMI 8226 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-155 RPMI 8226 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-155
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RPMI-7951 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-66 RPMI-7951 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-66
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RS4;11 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1873 RS4;11 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1873
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RSOI cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1419 RSOI cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1419
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RT4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-2 RT4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-2
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Ru Ra cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1315 Ru Ra cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1315
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RWPE-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11609 RWPE-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11609
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RWPE-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11610 RWPE-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11610
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
RWPE2-W99 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2853 RWPE2-W99 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2853
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Sal Mat cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1110 Sal Mat cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1110
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Saos-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-85 Saos-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-85
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Sar Nis cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1231 Sar Nis cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1231
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-9855 SC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-9855
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SC-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8756 SC-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8756
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SCaBER cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-3 SCaBER cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-3
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SCC-15 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1623 SCC-15 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1623
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SCC-25 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1628 SCC-25 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1628
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SCC-4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1624 SCC-4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1624
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SCC-9 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1629 SCC-9 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1629
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SH-4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7724 SH-4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7724
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SHM-D33 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1668 SHM-D33 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1668
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SHP-77 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2195 SHP-77 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2195
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SH-SY5Y cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2266 SH-SY5Y cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2266
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SiHa cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-35 SiHa cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-35
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SJCRH30 [RC13, RMS 13, SJRH30] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心CRL-2061 SJCRH30 [RC13, RMS 13, SJRH30] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心CRL-2061
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SJSA-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2098 SJSA-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2098
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-BR-3 [SKBR3] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-30 SK-BR-3 [SKBR3] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-30
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-CO-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-39 SK-CO-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-39
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-ES-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-86 SK-ES-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-86
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-HEP-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-52 SK-HEP-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-52
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-LMS-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-88 SK-LMS-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-88
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-LU-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-57 SK-LU-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-57
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-MEL-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-67 SK-MEL-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-67
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-MEL-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-68 SK-MEL-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-68
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-MEL-24 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-71 SK-MEL-24 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-71
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-MEL-28 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-72 SK-MEL-28 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-72
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-MEL-3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-69 SK-MEL-3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-69
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-MEL-31 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-73 SK-MEL-31 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-73
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-MEL-5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-70 SK-MEL-5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-70
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-MES-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-58 SK-MES-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-58
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-N-AS cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2137 SK-N-AS cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2137
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-N-BE(2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2271 SK-N-BE(2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2271
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-N-DZ cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2149 SK-N-DZ cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2149
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-NEP-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-48 SK-NEP-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-48
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-N-FI cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2142 SK-N-FI cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2142
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-N-MC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-10 SK-N-MC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-10
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-N-SH cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-11 SK-N-SH cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-11
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SKO-007 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8033-1 SKO-007 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8033-1
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-OV-3 [SKOV-3; SKOV3] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-77 SK-OV-3 [SKOV-3; SKOV3] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-77
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-PN-DW cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2139 SK-PN-DW cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2139
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-UT-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-114 SK-UT-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-114
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SK-UT-1B cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-115 SK-UT-1B cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-115
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SKW 6.4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-215 SKW 6.4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-215
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
sNF02.2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2885 sNF02.2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2885
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
sNF94.3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2886 sNF94.3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2886
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
sNF96.2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2884 sNF96.2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2884
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5971 SNU-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5971
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-16 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5974 SNU-16 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5974
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-182 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2235 SNU-182 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2235
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-387 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2237 SNU-387 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2237
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-398 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2233 SNU-398 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2233
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-423 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2238 SNU-423 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2238
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-449 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2234 SNU-449 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2234
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-475 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2236 SNU-475 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2236
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5973 SNU-5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5973
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-C1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5972 SNU-C1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-5972
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-C2A cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-250.1 SNU-C2A cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-250.1
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SNU-C2B cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-250 SNU-C2B cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-250
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SR cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2262 SR cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2262
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
ST486 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1647 ST486 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1647
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Stomach; Derived From Metastatic Site: Pleural Effusion And Supraclavicular And Axillary Lymph Nodes And Douglas Cul-De-Sac Stomach; Derived From Metastatic Site: Pleural Effusion And Supraclavicular And Axillary Lymph Nodes And Douglas Cul-De-Sac
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SU.86.86 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1837 SU.86.86 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1837
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SU-CCS-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2971 SU-CCS-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2971
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SU-DHL-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2955 SU-DHL-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2955
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SU-DHL-10 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2963 SU-DHL-10 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2963
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SU-DHL-16 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2964 SU-DHL-16 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2964
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SU-DHL-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2956 SU-DHL-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2956
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SU-DHL-4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2957 SU-DHL-4 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2957
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SU-DHL-5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2958 SU-DHL-5 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2958
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SU-DHL-8 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2961 SU-DHL-8 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2961
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SUP-B15 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1929 SUP-B15 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1929
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SUP-T1 [VB] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1942 SUP-T1 [VB] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1942
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SVG p12 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8621 SVG p12 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-8621
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SV-HUC-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-9520 SV-HUC-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-9520
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 1088 [SW-1088, SW1088] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-12 SW 1088 [SW-1088, SW1088] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-12
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 1271 [SW-1271, SW1271] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2177 SW 1271 [SW-1271, SW1271] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2177
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 1353 [SW 1353, SW-1353] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-94 SW 1353 [SW 1353, SW-1353] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-94
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 156 [SW-156, SW156] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2175 SW 156 [SW-156, SW156] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2175
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 1573 [SW-1573, SW1573] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2170 SW 1573 [SW-1573, SW1573] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2170
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 1783 [SW-1783, SW1783] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-13 SW 1783 [SW-1783, SW1783] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-13
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 1990 [SW-1990, SW1990] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2172 SW 1990 [SW-1990, SW1990] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2172
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 626 [SW-626, SW626] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-78 SW 626 [SW-626, SW626] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-78
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 684 [SW-684, SW684] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-91 SW 684 [SW-684, SW684] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-91
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 780 [SW-780, SW780] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2169 SW 780 [SW-780, SW780] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2169
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 872 [SW-872, SW872] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-92 SW 872 [SW-872, SW872] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-92
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 900 [SW-900, SW900] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-59 SW 900 [SW-900, SW900] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-59
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 954 [SW-954, SW954] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-117 SW 954 [SW-954, SW954] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-117
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW 982 [SW-982, SW982] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-93 SW 982 [SW-982, SW982] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-93
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW1116 [SW 1116, SW-1116] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-233 SW1116 [SW 1116, SW-1116] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-233
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW-13 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-105 SW-13 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-105
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW1417 [SW-1417] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-238 SW1417 [SW-1417] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-238
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW1463 [SW 1463, SW-1463] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-234 SW1463 [SW 1463, SW-1463] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-234
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW403 [SW-403] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-230 SW403 [SW-403] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-230
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW48 [SW-48] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-231 SW48 [SW-48] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-231
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW480 [SW-480] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-228 SW480 [SW-480] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-228
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW527 [SW 527, SW-527] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7940 SW527 [SW 527, SW-527] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7940
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW579 [SW 579, SW-579] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-107 SW579 [SW 579, SW-579] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-107
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW620 [SW-620] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-227 SW620 [SW-620] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-227
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW756 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-10302 SW756 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-10302
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW837 [SW-837] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-235 SW837 [SW-837] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-235
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW948 [SW-948] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-237 SW948 [SW-948] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-237
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
SW962 [SW 962, SW-962] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-118 SW962 [SW 962, SW-962] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-118
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
T/G HA-VSMC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1999 T/G HA-VSMC cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1999
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
T1 (174 x CEM.T1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1991 T1 (174 x CEM.T1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1991
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
T1-73 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7943 T1-73 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7943
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
T2 (174 x CEM.T2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1992 T2 (174 x CEM.T2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1992
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
T24 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-4 T24 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-4
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
T-47D cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-133 T-47D cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-133
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
T47D-KBluc cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2865 T47D-KBluc cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2865
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
T84 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-248 T84 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CCL-248
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
T98G [T98-G] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1690 T98G [T98-G] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1690
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tal Jo cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1267 Tal Jo cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1267
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TALL-104 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11386 TALL-104 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11386
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tau RD P301S FRET Biosensor cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3275 Tau RD P301S FRET Biosensor cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-3275
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TCCSUP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-5 TCCSUP cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-5
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TE 115.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7744 TE 115.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7744
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TE 125.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7945_FL TE 125.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7945_FL
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TE 130.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7746 TE 130.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7746
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TE 159.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7752 TE 159.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7752
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TE 353.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7761 TE 353.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7761
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TE 354.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7762 TE 354.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7762
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TE 381.T [RD114-B] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7763 TE 381.T [RD114-B] cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7763
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TE 441.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7767 TE 441.T cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7767
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TE 90.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7739 TE 90.Sk cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-7739
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tep Be cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1336 Tep Be cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-1336
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tera-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-105 Tera-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-105
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tera-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-106 Tera-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 HTB-106
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Testes; Derived From Metastatic Site: Lung Testes; Derived From Metastatic Site: Lung
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TF-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2003 TF-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2003
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TF-1.CN5a.1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2512 TF-1.CN5a.1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2512
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
TF-1a cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2451 TF-1a cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2451
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
THLE-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2706 THLE-2 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-2706
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
THLE-3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11233 THLE-3 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 CRL-11233
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
THP-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-202 THP-1 cell line细胞株及完全培养基-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 TIB-202
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Thyroid Thyroid
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Tissue:
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Adrenal Tissue: Adrenal
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Adrenal Gland Tissue: Adrenal Gland
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Adrenal Gland/Cortex Tissue: Adrenal Gland/Cortex
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Aorta/Smooth Muscle Tissue: Aorta/Smooth Muscle
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Ascites Tissue: Ascites
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Blood Tissue: Blood
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Bone Tissue: Bone
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Bone Marrow Tissue: Bone Marrow
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Bone Marrow/Stroma Tissue: Bone Marrow/Stroma
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Bone; Connective Tissue Tissue: Bone; Connective Tissue
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Bone; Derived From Metastatic Site: Lung Tissue: Bone; Derived From Metastatic Site: Lung
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain Tissue: Brain
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain (Lateral And Third Ventricle) Tissue: Brain (Lateral And Third Ventricle)
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain (Temporal Lobe) Tissue: Brain (Temporal Lobe)
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain Tumor From Posterior Fossa; At/Rt; Atrt Tissue: Brain Tumor From Posterior Fossa; At/Rt; Atrt
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain, Cerebral Cortex Tissue: Brain, Cerebral Cortex
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain, Cerebral Hemisphere Tissue: Brain, Cerebral Hemisphere
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain,&Nbsp; Derived From Metastatic Site:Pleural Fluid Tissue: Brain,&Nbsp; Derived From Metastatic Site:Pleural Fluid
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain/Brain Tumor Tissue: Brain/Brain Tumor
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain/Cerebrum; Right Temporal Lobe Tissue: Brain/Cerebrum; Right Temporal Lobe
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain/Right Frontal Parieto-Occipital Cortex Tissue: Brain/Right Frontal Parieto-Occipital Cortex
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain; Derived From Metastatic Site: Abdominal Mass Tissue: Brain; Derived From Metastatic Site: Abdominal Mass
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain; Derived From Metastatic Site: Bone Marrow Tissue: Brain; Derived From Metastatic Site: Bone Marrow
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Brain; Derived From Metastatic Site: Supra-Orbital Area Tissue: Brain; Derived From Metastatic Site: Supra-Orbital Area
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Breast Tissue: Breast
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Breast; Mammary Gland/Duct Tissue: Breast; Mammary Gland/Duct
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Breast; Mammary Gland/Duct;&Nbsp;Derived From Metastatic Site:&Nbsp;Lymph Node Tissue: Breast; Mammary Gland/Duct;&Nbsp;Derived From Metastatic Site:&Nbsp;Lymph Node
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Carcinoma; Classic Small Cell Lung Cancer; Lung Tissue: Carcinoma; Classic Small Cell Lung Cancer; Lung
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Cecum Tissue: Cecum
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Cervical Lymph Node Tissue: Cervical Lymph Node
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Cervix Tissue: Cervix
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Cervix; Derived From Metastatic Site: Lymph Node Tissue: Cervix; Derived From Metastatic Site: Lymph Node
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Cervix; Derived From Metastatic Site: Omentum Tissue: Cervix; Derived From Metastatic Site: Omentum
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Cervix; Derived From Metastatic Site: Small Intestine Tissue: Cervix; Derived From Metastatic Site: Small Intestine
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
Tissue: Cervix; Derived From Metastic Site: Lymph Node, Carcinoma Tissue: Cervix; Derived From Metastic Site: Lymph Node, Carcinoma
【Organism物种来源】人源Homo sapiens, human
【Tissue组织来源】
【Cell Type细胞类型】
【Product Format产品状态】 frozen/live culture
【Morphology细胞形态】
【Culture Properties细胞特性】
【Biosafety Level生物安全级别】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
【Disease细胞源疾病】
【Age年龄】
【Gender性别】 Male/Female
【Storage Conditions保存条件】 liquid nitrogen vapor phase
【Karyotype染色体组型】
【Images细胞图片】 Cell Micrograph
【Derivation衍生细胞】
【Clinical Data临床数据】
【Comments其他描述】
【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing传代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation冻存条件】
【Freeze Medium冻存培养基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存温度】 Liquid nitrogen vapor phase
【Culture Conditions培养条件】
【Atmosphere培养环境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培养温度】 37°C
【STR Profile图谱】
【Population Doub领 Time倍增殖时间】
【References参考文献】
【Supplier细胞供应厂家】BioVector NTCC Inc.
【Website网址】http://www.biovector.net
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