鸡蛋中的氨基糖苷分析

Aminoglycosides in Eggs

Conditions
Column: ACE Excel 2 C18-PFP
Dimensions: 100 x 2.1 mm
Part Number: EXL-1010-1002U
Mobile Phase:

A: 20 mM HFBA in H2O/MeCN (98:2 v/v)
B: 20 mM HFBA in MeCN/H2O (98:2 v/v)

Time(mins)	%B	Curve
0.0	5.0	-
2.0	15.0	6
4.5	19.0	6
5.5	19.5	8
6.0	22.0	6
7.0	35.0	6
9.0	48.0	8
9.5	5.0	6

Flow Rate: 0.4 mL/min
Temperature: 40 °C
Detection: Positive ESI MRM (transitions as shown)
Sample: Extraction at low pH, clean up with WCX SPE cartridge
Egg sample spiked at 100 μg/kg (CCα)

Key
GNT Gentamicin
TBM Tobramycin
KNM Kanamycin
DHS Dihydrostreptomycin
STR Streptomycin
HGB Higromycin-B
SPT Spectinomycin