[ACE UltraCore SuperC18色谱柱]人参皂

[ACE UltraCore SuperC18色谱柱]人参皂甙分析

Conditions
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 100 x 3.0 mm; 2 x 100 x 3.0 mm (coupled); 3 x 100 x 3.0 mm (coupled)
Part Number: CORE-25A-1003U
Mobile Phase: 

A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN

Gradient:

Flow Rate: 0.8 mL/min
Injection: 2 μL (100 x 3.0 mm); 4 μL (200 x 3.0 mm); 6 μL (300 x 3.0 mm)
Temperature: 80 °C
Detection: UV, 203 nm
Sample: 5 x 75 mg tablets ground to fine powder and extracted with 10.0 mL MeCN/H2O (1:1 v/v) for 15 minutes with ultrasonication. 100 μL supernatant diluted with 300 μL water and filtered using a Whatman Mini-Uniprep syringeless filter
System: Chromaster Ultra Rs